Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus

A. I. Glukhov, S. A. Gordeev, S. V. Vinogradov, V. I. Kiselev, V. M. Kramarov, O. I. Kiselev, E. S. Severin

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Abstract

Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.

Original languageEnglish (US)
Pages (from-to)435-443
Number of pages9
JournalMolecular and Cellular Probes
Volume4
Issue number6
DOIs
StatePublished - Dec 1990

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Keywords

  • DNA polymerase
  • DNA probes for Epstein-Barr virus
  • polymerase chain reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Glukhov, A. I., Gordeev, S. A., Vinogradov, S. V., Kiselev, V. I., Kramarov, V. M., Kiselev, O. I., & Severin, E. S. (1990). Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus. Molecular and Cellular Probes, 4(6), 435-443. https://doi.org/10.1016/0890-8508(90)90002-H