Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus

A. I. Glukhov, S. A. Gordeev, S. V. Vinogradov, V. I. Kiselev, V. M. Kramarov, O. I. Kiselev, E. S. Severin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.

Original languageEnglish (US)
Pages (from-to)435-443
Number of pages9
JournalMolecular and Cellular Probes
Volume4
Issue number6
DOIs
StatePublished - Dec 1990

Fingerprint

Human Herpesvirus 4
Thermus
HIV
Replicon
DNA Primers
DNA-Directed DNA Polymerase
Viruses
Polymerase Chain Reaction
DNA
Thermus thermophilus DNA polymerase

Keywords

  • DNA polymerase
  • DNA probes for Epstein-Barr virus
  • polymerase chain reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Glukhov, A. I., Gordeev, S. A., Vinogradov, S. V., Kiselev, V. I., Kramarov, V. M., Kiselev, O. I., & Severin, E. S. (1990). Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus. Molecular and Cellular Probes, 4(6), 435-443. https://doi.org/10.1016/0890-8508(90)90002-H

Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus. / Glukhov, A. I.; Gordeev, S. A.; Vinogradov, S. V.; Kiselev, V. I.; Kramarov, V. M.; Kiselev, O. I.; Severin, E. S.

In: Molecular and Cellular Probes, Vol. 4, No. 6, 12.1990, p. 435-443.

Research output: Contribution to journalArticle

Glukhov, AI, Gordeev, SA, Vinogradov, SV, Kiselev, VI, Kramarov, VM, Kiselev, OI & Severin, ES 1990, 'Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus', Molecular and Cellular Probes, vol. 4, no. 6, pp. 435-443. https://doi.org/10.1016/0890-8508(90)90002-H
Glukhov, A. I. ; Gordeev, S. A. ; Vinogradov, S. V. ; Kiselev, V. I. ; Kramarov, V. M. ; Kiselev, O. I. ; Severin, E. S. / Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus. In: Molecular and Cellular Probes. 1990 ; Vol. 4, No. 6. pp. 435-443.
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