Ammonia causes decreased brain monoamines in fathead minnows (Pimephales promelas)

Patrick Ronan, Mark P. Gaikowski, Steven J. Hamilton, Kevin J. Buhl, Cliff H. Summers

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Hyperammonemia, arising from variety of disorders, leads to severe neurological dysfunction. The mechanisms of ammonia toxicity in brain are not completely understood. This study investigated the effects of ammonia on monoaminergic systems in brains of fathead minnows (Pimephales promelas). Fish serve as a good model system to investigate hyperammonemic effects on brain function since no liver manipulations are necessary to increase endogenous ammonia concentrations. Using high performance liquid chromatography with electrochemical detection, monoamines and some associated metabolites were measured from whole brain homogenate. Adult males were exposed for 48 h to six different concentrations of ammonia (0.01-2.36 mg/l unionized) which bracketed the 96-h LC50 for this species. Ammonia concentration-dependent decreases were found for the catecholamines (norepinephrine and dopamine) and the indoleamine serotonin (5-HT). After an initial increase in the 5-HT precursor 5-hydroxytryptophan it too decreased with increasing ammonia concentrations. There were also significant increases in the 5-HIAA/5-HT and DOPAC/DA ratios, often used as measures of turnover. There were no changes in epinephrine (Epi) or monoamine catabolites (DOPAC, 5-HIAA) at any ammonia concentrations tested. Results suggest that ammonia causes decreased synthesis while also causing increased release and degradation. Increased release may underlie behavioral reactions to ammonia exposure in fish. This study adds weight to a growing body of evidence demonstrating that ammonia leads to dysfunctional monoaminergic systems in brain which may underlie neurological symptoms associated with human disorders such as hepatic encephalopathy.

Original languageEnglish (US)
Pages (from-to)184-191
Number of pages8
JournalBrain Research
Volume1147
Issue number1
DOIs
StatePublished - May 25 2007

Fingerprint

Cyprinidae
Ammonia
Brain
Serotonin
3,4-Dihydroxyphenylacetic Acid
Hydroxyindoleacetic Acid
Fishes
Hyperammonemia
5-Hydroxytryptophan
Hepatic Encephalopathy
Epinephrine
Catecholamines
Dopamine
Norepinephrine
High Pressure Liquid Chromatography
Weights and Measures

Keywords

  • Dopamine
  • Epinephrine
  • Fish
  • Norepinephrine
  • Serotonin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Developmental Biology
  • Clinical Neurology

Cite this

Ammonia causes decreased brain monoamines in fathead minnows (Pimephales promelas). / Ronan, Patrick; Gaikowski, Mark P.; Hamilton, Steven J.; Buhl, Kevin J.; Summers, Cliff H.

In: Brain Research, Vol. 1147, No. 1, 25.05.2007, p. 184-191.

Research output: Contribution to journalArticle

Ronan, Patrick ; Gaikowski, Mark P. ; Hamilton, Steven J. ; Buhl, Kevin J. ; Summers, Cliff H. / Ammonia causes decreased brain monoamines in fathead minnows (Pimephales promelas). In: Brain Research. 2007 ; Vol. 1147, No. 1. pp. 184-191.
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