Amino acid sequence of the active site of human serum cholinesterase from usual, atypical, and atypical-silent genotypes

Oksana Lockridge, Bert N. La Du

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Active-site tryptic peptides were isolated from three genetic types of human serum cholinesterase. The active-site peptide was identified by labeling the active-site serine with [3H] diisopropylfluorophosphate. Peptides were purified by high-performance liquid chromatography. Amino acid composition and sequence analysis showed that the peptide from the usual genotype contained 29 residues with the sequence Ser-Val-Thr-Leu-Phe-Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser-Val-Ser-Leu-His-Leu-Leu-Ser-Pro-Gly-Ser-His-Ser-Leu-Phe-Thr-Arg. The active-site serine was the eighth residue from the N- terminal. The peptide containing the active-site serine from the atypical genotype contained 22 residues with the sequence Ser-Val-Thr-Leu-Phe-Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser-Val-Ser-Leu-His-Leu-Leu-Ser-Pro-Gly. The peptide from the atypical-silent genotype contained eight residues with the sequence Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser. Thus, the sequences of the atypical and atypical-silent active-site peptides were identical to the corresponding portions of the usual peptide.

Original languageEnglish (US)
Pages (from-to)485-498
Number of pages14
JournalBiochemical Genetics
Volume24
Issue number5-6
DOIs
StatePublished - Jun 1 1986

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cholinesterase
Cholinesterases
active sites
peptide
serum
Amino Acid Sequence
Catalytic Domain
genotype
amino acid sequences
amino acid
Genotype
peptides
Amino Acids
Peptides
Serum
serine
Serine
Isoflurophate
Protein Sequence Analysis
Medical Genetics

Keywords

  • active-site sequence
  • cholinesterase

ASJC Scopus subject areas

  • Biochemistry
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Amino acid sequence of the active site of human serum cholinesterase from usual, atypical, and atypical-silent genotypes. / Lockridge, Oksana; La Du, Bert N.

In: Biochemical Genetics, Vol. 24, No. 5-6, 01.06.1986, p. 485-498.

Research output: Contribution to journalArticle

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