Amino Acid Sequence of Escherichia coli Alkaline Phosphatase. Amino- and Carboxyl-Terminal Sequences and Variations between Two Isozymes

Philip M Kelley, Peter A. Neumann, Karen Shriefer, Fiorella Cancedda, Milton J. Schlesinger, Ralph A. Bradshaw

Research output: Contribution to journalArticle

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Abstract

The amino- and carboxyl-terminal fragments of E. coli alkaline phosphatase, produced by cyanogen bromide cleavage, have been isolated. The carboxyl-terminal fragment, CB-VI, contains seven amino acid residues in the sequence Lys-Ala-Ala-Leu-Gly-Leu-Lys. The absence of homo-serine and the coincidence of carboxyl-terminal lysine in native alkaline phosphatase suggest the carboxyl-terminal assignment. The amino-terminal fragment, CB-VII, contains five and four residues when isolated from isozymes 1 and 3, respectively. CB-VII from isozyme 1 possesses the sequence Arg-Thr-Pro-Glu-HSe. The corresponding peptide from isozyme 3 is devoid of the argininyl residue. Amino-terminal analyses of native alkaline phosphatase suggest the amino-terminal alignment of CB-VII. Analyses for carbohydrate indicate both isozymes are devoid of those sugars commonly found in glycoproteins.

Original languageEnglish (US)
Pages (from-to)3499-3503
Number of pages5
JournalBiochemistry
Volume12
Issue number18
DOIs
StatePublished - Aug 1 1973

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Escherichia coli
Isoenzymes
Alkaline Phosphatase
Amino Acid Sequence
Amino Acids
Cyanogen Bromide
Sugars
Serine
Lysine
Glycoproteins
Carbohydrates
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kelley, P. M., Neumann, P. A., Shriefer, K., Cancedda, F., Schlesinger, M. J., & Bradshaw, R. A. (1973). Amino Acid Sequence of Escherichia coli Alkaline Phosphatase. Amino- and Carboxyl-Terminal Sequences and Variations between Two Isozymes. Biochemistry, 12(18), 3499-3503. https://doi.org/10.1021/bi00742a023

Amino Acid Sequence of Escherichia coli Alkaline Phosphatase. Amino- and Carboxyl-Terminal Sequences and Variations between Two Isozymes. / Kelley, Philip M; Neumann, Peter A.; Shriefer, Karen; Cancedda, Fiorella; Schlesinger, Milton J.; Bradshaw, Ralph A.

In: Biochemistry, Vol. 12, No. 18, 01.08.1973, p. 3499-3503.

Research output: Contribution to journalArticle

Kelley, PM, Neumann, PA, Shriefer, K, Cancedda, F, Schlesinger, MJ & Bradshaw, RA 1973, 'Amino Acid Sequence of Escherichia coli Alkaline Phosphatase. Amino- and Carboxyl-Terminal Sequences and Variations between Two Isozymes', Biochemistry, vol. 12, no. 18, pp. 3499-3503. https://doi.org/10.1021/bi00742a023
Kelley, Philip M ; Neumann, Peter A. ; Shriefer, Karen ; Cancedda, Fiorella ; Schlesinger, Milton J. ; Bradshaw, Ralph A. / Amino Acid Sequence of Escherichia coli Alkaline Phosphatase. Amino- and Carboxyl-Terminal Sequences and Variations between Two Isozymes. In: Biochemistry. 1973 ; Vol. 12, No. 18. pp. 3499-3503.
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