Amelioration of cytotoxic effects of idarubicin and 4HC on Chang liver cells by a novel grape seed proanthocyanidin extract

Shantaram S Joshi, E. J. Benner, J. Balmoori, D. Bagchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Despite anticancer chemotherapeutic agents are very effective in inhibiting growth of cancer cells in vitro and in vivo, toxicity to normal cells is a major problem. In an attempt to ameliorate the toxic effects in this study we have used a novel grape seed proanthocyanidin extract (GSPE) generously provided by InterHealth Nutritionals, Concord, CA. Chang liver cells, established from non-malignant human tissue, were treated in vitro with idarubicin (30 nM) and 4- hydroxyperoxycyclophosphamide (4HC) (1 μg/ml) with or without GSPE (25 μg/ml). The cells were grown in vitro and the growth rate of the cells were determined using MTT assay. Our results showed that the GSPE decreased growth inhibitory effects of idarubicin and 4HC on Chang liver cells in vitro. Since these chemotherapeutic agents are known to induce apoptosis in the target cells, we also looked for the expression of one of the apoptosis related gene bcl2 in the treated and control cells using Western blotting techniques. There was an increased expression of bcl2 in the cells treated with GSPE. Thus, these results indicate that GSPE can be a potential candidate to ameliorate the toxic effects associated with chemotherapeutic agents.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

Fingerprint

idarubicin
Grape Seed Extract
Idarubicin
grape seeds
proanthocyanidins
Liver
hepatocytes
cytotoxicity
extracts
Poisons
cells
Apoptosis
apoptosis
antineoplastic agents
Antineoplastic Agents
Toxicity
Growth
Assays
Genes
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Amelioration of cytotoxic effects of idarubicin and 4HC on Chang liver cells by a novel grape seed proanthocyanidin extract. / Joshi, Shantaram S; Benner, E. J.; Balmoori, J.; Bagchi, D.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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