Alu-polymerase chain reaction genomic fingerprinting technique identifies multiple genetic loci associated with pancreatic tumourigenesis

Arthur B. McKie, Takeshi Iwamura, Hing Y. Leung, Michael A Hollingsworth, Nicholas R. Lemoine

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

DNA fingerprinting can be used to detect genetic rearrangements in cancer that may be associated with activation of oncogenes and inactivation of tumour suppressor genes. We have developed a fingerprinting strategy based on polymerase chain reaction (PCR) amplification of genomic DNA with primers specific for the Alu repeat sequences, which are highly abundant in the human genome. This has been applied to DNA from pancreatic cancer and paired normal samples to isolate and identify fragments of genomic DNA rearranged in the malignant cells. These fragments have been sequenced and used as probes to isolate hybridising clones from gridded bacteriophage PI, phage artificial chromosome, and cosmid libraries for fluorescent in situ hybridisation mapping and the identification of expressed sequences. Further characterisation has identified a putative novel gene (ARTI) that is up- regulated specifically in pancreatic cancer as well as another sequence with similarity to genes involved in differentiation (POU domains). In conclusion, we suggest that Alu-PCR fingerprinting may be a useful technique for the identification of genes involved in tumourigenesis.

Original languageEnglish (US)
Pages (from-to)30-41
Number of pages12
JournalGenes Chromosomes and Cancer
Volume18
Issue number1
DOIs
StatePublished - Jan 18 1997

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Genetic Loci
Pancreatic Neoplasms
Polymerase Chain Reaction
Bacteriophages
Artificial Chromosomes
Genes
Cosmids
DNA Primers
DNA Fingerprinting
DNA
Human Genome
Tumor Suppressor Genes
Fluorescence In Situ Hybridization
Oncogenes
Clone Cells
Neoplasms

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Alu-polymerase chain reaction genomic fingerprinting technique identifies multiple genetic loci associated with pancreatic tumourigenesis. / McKie, Arthur B.; Iwamura, Takeshi; Leung, Hing Y.; Hollingsworth, Michael A; Lemoine, Nicholas R.

In: Genes Chromosomes and Cancer, Vol. 18, No. 1, 18.01.1997, p. 30-41.

Research output: Contribution to journalArticle

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