AltTrans: Transcript pattern variants annotated for both alternative splicing and alternative polyadenylation

Vincent Le Texier, Jean Jack Riethoven, Vasudev Kumanduri, Chellappa Gopalakrishnan, Fabrice Lopez, Daniel Gautheret, Thangavel Alphonse Thanaraj

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: The three major mechanisms that regulate transcript formation involve the selection of alternative sites for transcription start (TS), splicing, and polyadenylation. Currently there are efforts that collect data & annotation individually for each of these variants. It is important to take an integrated view of these data sets and to derive a data set of alternate transcripts along with consolidated annotation. We have been developing in the past computational pipelines that generate value-added data at genome-scale on individual variant types; these include AltSplice on splicing and AltPAS on polyadenylation. We now extend these pipelines and integrate the resultant data sets to facilitate an integrated view of the contributions from splicing and polyadenylation in the formation of transcript variants. Description: The AltSplice pipeline examines gene- transcript alignments and delineates alternative splice events and splice patterns; this pipeline is extended as AltTrans to delineate isoform transcript patterns for each of which both introns/exons and 'terminating' polyA site are delineated; EST/mRNA sequences that qualify the transcript pattern confirm both the underlying splicing and polyadenylation. The AltPAS pipeline examines gene-transcript alignments and delineates all potential polyA sites irrespective of underlying splicing patterns. Resultant polyA sites from both AltTrans and AltPAS are merged. The generated database reports data on alternative splicing, alternative polyadenylation and the resultant alternate transcript patterns; the basal data is annotated for various biological features. The data (named as integrated AltTrans data) generated for both the organisms of human and mouse is made available through the Alternate Transcript Diversity web site at http://www.ebi.ac.uk/atd/. Conclusion: The reported data set presents alternate transcript patterns that are annotated for both alternative splicing and alternative polyadenylation. Results based on current transcriptome data indicate that the contribution of alternative splicing is larger than that of alternative polyadenylation.

Original languageEnglish (US)
Article number169
JournalBMC bioinformatics
Volume7
DOIs
StatePublished - Mar 23 2006

Fingerprint

Alternative Splicing
Polyadenylation
Pipelines
Alternatives
Genes
Alternate
Transcription Initiation Site
Expressed Sequence Tags
Transcription
Introns
Websites
Exons
Protein Isoforms
Annotation
Alignment
Transcriptome
Messenger RNA
Gene
Genome
Databases

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Applied Mathematics

Cite this

AltTrans : Transcript pattern variants annotated for both alternative splicing and alternative polyadenylation. / Le Texier, Vincent; Riethoven, Jean Jack; Kumanduri, Vasudev; Gopalakrishnan, Chellappa; Lopez, Fabrice; Gautheret, Daniel; Thanaraj, Thangavel Alphonse.

In: BMC bioinformatics, Vol. 7, 169, 23.03.2006.

Research output: Contribution to journalArticle

Le Texier, Vincent ; Riethoven, Jean Jack ; Kumanduri, Vasudev ; Gopalakrishnan, Chellappa ; Lopez, Fabrice ; Gautheret, Daniel ; Thanaraj, Thangavel Alphonse. / AltTrans : Transcript pattern variants annotated for both alternative splicing and alternative polyadenylation. In: BMC bioinformatics. 2006 ; Vol. 7.
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