Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani

Bonnie A. McNeil, Dawn M. Simon, Steven Zimmerly

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Group II introns are ribozymes and retroelements found in bacteria, and are thought to have been the ancestors of nuclear pre-mRNA introns. Whereas nuclear introns undergo prolific alternative splicing in some species, group II introns are not known to carry out equivalent reactions. Here we report a group II intron in the human pathogen Clostridium tetani, which undergoes four alternative splicing reactions in vivo. Together with unspliced transcript, five mRNAs are produced, each encoding a distinct surface layer protein isoform. Correct fusion of exon reading frames requires a shifted 5′ splice site located 8 nt upstream of the canonical boundary motif. The shifted junction is accomplished by an altered IBS1-EBS1 pairing between the intron and 5′ exon. Growth of C. tetani under a variety of conditions did not result in large changes in alternative splicing levels, raising the possibility that alternative splicing is constitutive. This work demonstrates a novel type of gene organization and regulation in bacteria, and provides an additional parallel between group II and nuclear pre-mRNA introns.

Original languageEnglish (US)
Pages (from-to)1959-1969
Number of pages11
JournalNucleic acids research
Volume42
Issue number3
DOIs
StatePublished - Feb 1 2014

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Clostridium tetani
Alternative Splicing
Introns
Genes
RNA Precursors
Exons
Bacteria
Reading Frames
Catalytic RNA
Retroelements
RNA Splice Sites
S-layer proteins
Protein Isoforms
Messenger RNA

ASJC Scopus subject areas

  • Genetics

Cite this

Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani. / McNeil, Bonnie A.; Simon, Dawn M.; Zimmerly, Steven.

In: Nucleic acids research, Vol. 42, No. 3, 01.02.2014, p. 1959-1969.

Research output: Contribution to journalArticle

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