Alternate splicing of the rTS gene product and its overexpression in a 5-fluorouracil-resistant cell line

Bruce J. Dolnick, Adrian R Black

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

rTS is a gene with convergent and overlapping transcription relative to thymidylate synthase (TS). Screening of a cDNA library has identified a second rTS RNA (rTSβ) which is related to a previously described rTS RNA (rTSα). rTSβ differs from rTSα by the insertion of an extra 116 nucleotides after base 128 of rTSα and an altered 3'-noncoding region such that rTSβ RNA is not complementary to TS mRNA. rTSα and rTSβ RNAs have open reading frames predicted to encode proteins of 361 and 416 amino acids, respectively. Antibody to rTSβ protein detects two proteins of the predicted molecular weight in the H630 colon tumor cell line. rTSβ is overexpressed 40-70-fold in a 5-fluorouracil-resistant H630 subline compared with 7-8-fold for TS and rTSα.

Original languageEnglish (US)
Pages (from-to)3207-3210
Number of pages4
JournalCancer Research
Volume56
Issue number14
StatePublished - Jul 15 1996

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Alternative Splicing
Fluorouracil
Thymidylate Synthase
RNA
Cell Line
Genes
Proteins
Tumor Cell Line
Gene Library
Open Reading Frames
Colon
Nucleotides
Molecular Weight
Amino Acids
Messenger RNA
Antibodies

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Alternate splicing of the rTS gene product and its overexpression in a 5-fluorouracil-resistant cell line. / Dolnick, Bruce J.; Black, Adrian R.

In: Cancer Research, Vol. 56, No. 14, 15.07.1996, p. 3207-3210.

Research output: Contribution to journalArticle

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