Altered theca and cumulus oocyte complex gene expression, follicular arrest and reduced fertility in cows with dominant follicle follicular fluid androgen excess

Adam F. Summers, William E. Pohlmeier, Kevin M. Sargent, Brizett D. Cole, Rebecca J. Vinton, Scott G. Kurz, Renee M. McFee, Robert A. Cushman, Andrea S. Cupp, Jennifer R. Wood

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aspiration of bovine follicles 12-36 hours after induced corpus luteum lysis serendipitously identified two populations of cows, one with High androstenedione (A4; >40 ng/ml; mean = 102) and another with Low A4 (<20 ng/ml; mean = 9) in follicular fluid. We hypothesized that the steroid excess in follicular fluid of dominant follicles in High A4 cows would result in reduced fertility through altered follicle development and oocyte maternal RNA abundance. To test this hypothesis, estrous cycles of cows were synchronized and ovariectomy was performed 36 hours later. HPLC MS/MS analysis of follicular fluid showed increased dehydroepiandrosterone (6-fold), A4 (158-fold) and testosterone (31-fold) in the dominant follicle of High A4 cows. However, estrone (3-fold) and estradiol (2-fold) concentrations were only slightly elevated, suggesting a possible inefficiency in androgen to estrogen conversion in High A4 cows. Theca cell mRNA expression of LHCGR, GATA6, CYP11A1, and CYP17A1 was greater in High A4 cows. Furthermore, abundance of ZAR1 was decreased 10-fold in cumulus oocyte complexes from High A4 cows, whereas NLRP5 abundance tended to be 19.8-fold greater (P = 0.07). There was a tendency for reduction in stage 4 follicles in ovarian cortex samples from High A4 cows suggesting that progression to antral stages were impaired. High A4 cows tended (P<0.07) to have a 17% reduction in calving rate compared with Low A4 cows suggesting reduced fertility in the High A4 population. These data suggest that the dominant follicle environment of High A4 cows including reduced estrogen conversion and androgen excess contributes to infertility in part through altered follicular and oocyte development.

Original languageEnglish (US)
Article numbere110683
JournalPloS one
Volume9
Issue number10
DOIs
StatePublished - Oct 16 2014

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Follicular Fluid
follicular fluid
androgens
Gene expression
Androgens
Oocytes
Fertility
oocytes
Gene Expression
cows
gene expression
Fluids
Estrogens
Cholesterol Side-Chain Cleavage Enzyme
Theca Cells
Ovarian Follicle
Estrous Cycle
Dehydroepiandrosterone
Androstenedione
Estrone

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Altered theca and cumulus oocyte complex gene expression, follicular arrest and reduced fertility in cows with dominant follicle follicular fluid androgen excess. / Summers, Adam F.; Pohlmeier, William E.; Sargent, Kevin M.; Cole, Brizett D.; Vinton, Rebecca J.; Kurz, Scott G.; McFee, Renee M.; Cushman, Robert A.; Cupp, Andrea S.; Wood, Jennifer R.

In: PloS one, Vol. 9, No. 10, e110683, 16.10.2014.

Research output: Contribution to journalArticle

Summers, Adam F. ; Pohlmeier, William E. ; Sargent, Kevin M. ; Cole, Brizett D. ; Vinton, Rebecca J. ; Kurz, Scott G. ; McFee, Renee M. ; Cushman, Robert A. ; Cupp, Andrea S. ; Wood, Jennifer R. / Altered theca and cumulus oocyte complex gene expression, follicular arrest and reduced fertility in cows with dominant follicle follicular fluid androgen excess. In: PloS one. 2014 ; Vol. 9, No. 10.
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