Altered dendritic cell phenotype in response to Leishmania amazonensis amastigote infection is mediated by MAP kinase, ERK

Paola Mercedes Boggiatto, Fei Jie, Mousumi Ghosh, Katherine Nicole Gibson-Corley, Amanda Ramer-Tait, Douglas Elliot Jones, Christine Anne Petersen

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Initiation of productive immune responses against Leishmania depends on the successful transition of dendritic cells (DC) from an immature to a mature phenotype. This process is characterized by high CD40 surface expression as well as interleukin-12 production, which are frequently seen in response to L. major infection. In vivo footpad infection of C3HeB/FeJ mice for 7 days with L. amazonensis promoted an immature CD11c+ DC phenotype characterized by both significantly low CD40 surface expression and significantly decreased interleukin-12p40 production compared with L. major infection of these same mice. In vitro infection of bone marrow-derived dendritic cells with L. amazonensis amastigotes resulted in rapid and significant phosphorylation of the mitogen activated protein kinase, extracellular signal-regulated kinase 1/2, observed within minutes of exposure to the parasite. Infection with L. amazonensis promastigotes led to increased 1/2 phosphorylation after 4 hours of infection compared with L. major infection, which correlated with promastigote transformation into amastigotes. Treatment of bone marrow-derived dendritic cells with a mitogen activated protein kinase kinase-specific inhibitor, PD98059, led to regained surface CD40 expression and interleukin-12p40 production following L. amazonensis amastigote infection compared with non-treated, infected DC. Treatment of L. amazonensis-infected mice with the highly-specific mitogen activated protein kinase kinase inhibitor, CI-1040, enhanced surface CD40 expression on CD11c+ DC obtained from the draining lymph node. L. amazonensis amastigotes, through activation of extracellular signal-regulated kinase 1/2, inhibit the ability of DC to undergo proper maturation both in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)1818-1826
Number of pages9
JournalAmerican Journal of Pathology
Volume174
Issue number5
DOIs
StatePublished - Jan 1 2009

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Leishmania
Dendritic Cells
Phosphotransferases
Phenotype
Infection
Mitogen-Activated Protein Kinase 3
Interleukins
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase Kinases
Bone Marrow
Phosphorylation
Interleukin-12
Mitogen-Activated Protein Kinases
Parasites
Lymph Nodes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Altered dendritic cell phenotype in response to Leishmania amazonensis amastigote infection is mediated by MAP kinase, ERK. / Boggiatto, Paola Mercedes; Jie, Fei; Ghosh, Mousumi; Gibson-Corley, Katherine Nicole; Ramer-Tait, Amanda; Jones, Douglas Elliot; Petersen, Christine Anne.

In: American Journal of Pathology, Vol. 174, No. 5, 01.01.2009, p. 1818-1826.

Research output: Contribution to journalArticle

Boggiatto, Paola Mercedes ; Jie, Fei ; Ghosh, Mousumi ; Gibson-Corley, Katherine Nicole ; Ramer-Tait, Amanda ; Jones, Douglas Elliot ; Petersen, Christine Anne. / Altered dendritic cell phenotype in response to Leishmania amazonensis amastigote infection is mediated by MAP kinase, ERK. In: American Journal of Pathology. 2009 ; Vol. 174, No. 5. pp. 1818-1826.
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