Altered colony‐forming activities of bone marrow hematopoietic stem cells in mice following short‐term in vivo exposure to parathion

Vincent S. Gallicchio, Thomas D. Watts, George P. Casale, Philip M. Bartholomew

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This paper describes a study of hematopoiesis in parathion‐treated mice. Adult mice (48 C57B1/6) were given a daily dose of parathion (4 mg/kg p.o.) or corn oil vehicle (5 ml/kg p.o.) for 14 days. During the pesticide and the examination period, treated animals showed no signs of poisoning and had normal body weights. On days 2, 5, 7, 9, 12 and 14 following parathion or corn oil, femoral marrow cells were assayed in vitro for granulocyte/monocyte (CFU‐gm), erythroid (CFU‐e and BFU‐e), megakaryocyte (CFU‐meg), stromal (CFU‐str) and multipotential (CFU‐mix) hematopoietic stem cells. Leukocyte counts were elevated on days 2 and 5, while platelet counts were not increased until day 12. No change was observed in either hematocrits or numbers of marrow cells. BFU‐e were reduced (23% of control) by day 7, then increased to 137% of control by day 14. CFU‐e were reduced (41% of control) on day 9, then increased to 71% of control by day 14. CFU‐mix were 130% of control (day 2), then declined to control values by day 5. On days 12 and 14, CFU‐mix colonies decreased to 40% of control. CFU‐str were reduced at all time points examined. CFU‐gm were 123%, 136% and 130% of control on days 7, 12 and 14, respectively, while CFU‐meg were increased (145% of control) on day 7. The data suggest that parathion alters the cloning potential of bone marrow precursor stem cells.

Original languageEnglish (US)
Pages (from-to)231-241
Number of pages11
JournalThe International Journal of Cell Cloning
Volume5
Issue number3
DOIs
StatePublished - 1987

Fingerprint

Parathion
Hematopoietic Stem Cells
Corn Oil
Megakaryocytes
Bone Marrow
Ideal Body Weight
Hematopoiesis
Thigh
Platelet Count
Leukocyte Count
Hematocrit
Granulocytes
Pesticides
Poisoning
Organism Cloning
Monocytes
Stem Cells
Cell Count

Keywords

  • Hematopoietic toxicity
  • Organophosphates
  • Parathion
  • Stem cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Altered colony‐forming activities of bone marrow hematopoietic stem cells in mice following short‐term in vivo exposure to parathion. / Gallicchio, Vincent S.; Watts, Thomas D.; Casale, George P.; Bartholomew, Philip M.

In: The International Journal of Cell Cloning, Vol. 5, No. 3, 1987, p. 231-241.

Research output: Contribution to journalArticle

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abstract = "This paper describes a study of hematopoiesis in parathion‐treated mice. Adult mice (48 C57B1/6) were given a daily dose of parathion (4 mg/kg p.o.) or corn oil vehicle (5 ml/kg p.o.) for 14 days. During the pesticide and the examination period, treated animals showed no signs of poisoning and had normal body weights. On days 2, 5, 7, 9, 12 and 14 following parathion or corn oil, femoral marrow cells were assayed in vitro for granulocyte/monocyte (CFU‐gm), erythroid (CFU‐e and BFU‐e), megakaryocyte (CFU‐meg), stromal (CFU‐str) and multipotential (CFU‐mix) hematopoietic stem cells. Leukocyte counts were elevated on days 2 and 5, while platelet counts were not increased until day 12. No change was observed in either hematocrits or numbers of marrow cells. BFU‐e were reduced (23{\%} of control) by day 7, then increased to 137{\%} of control by day 14. CFU‐e were reduced (41{\%} of control) on day 9, then increased to 71{\%} of control by day 14. CFU‐mix were 130{\%} of control (day 2), then declined to control values by day 5. On days 12 and 14, CFU‐mix colonies decreased to 40{\%} of control. CFU‐str were reduced at all time points examined. CFU‐gm were 123{\%}, 136{\%} and 130{\%} of control on days 7, 12 and 14, respectively, while CFU‐meg were increased (145{\%} of control) on day 7. The data suggest that parathion alters the cloning potential of bone marrow precursor stem cells.",
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