Altered activities of hepatic and extrahepatic microsomal mixed function oxidase enzymes in diabetic and adrenalectomized diabetic rats

Walid A. Al-Turk, Sidney J. Stohs, Edward B. Roche

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Microsomal aryl hydrocarbon hydroxylase (AHH) and 7-ethoxycoumarin-O-deethylase (ECD) activities were determined in liver, lung and intestine of control, streptozotocin(STZ)-diabetic, and diabetic-adrenalectomized male and female rats. Hepatic cytochrome P-450 content was also determined. The diabetic state reduced hepatic AHH activity and increased ECD activity in control male rats, and failed to do so in the adrenalectomized male rats. The diabetic state increased hepatic AHH and ECD activities in the control female rats, while in the adrenalectomized female rats the activities of both enzymes were decreased compared to the control diabetic rats. STZ-induced diabetes produced a decrease in pulmonary AHH and ECD activities and increased intestinal AHH and ECD activities in both sexes. The diabetic state in the adrenalectomized rats resulted in further reduction in pulmonary AHH and ECD activities in both sexes, but failed to increase the intestinal AHH activity only in the female rat. Hepatic cytochrome P-450 contents were increased in the female but not male adrenalectomized rats when treated with STZ. The effects of STZ-induced diabetes in adrenalectomized rats on AHH. ECD and hepatic cytochrome P-450 content in liver, lung and intestine depended upon sex of animal, substrate and tissue.

Original languageEnglish (US)
Pages (from-to)337-345
Number of pages9
JournalPharmacology
Volume23
Issue number6
DOIs
StatePublished - Jan 1 1981

Fingerprint

Aryl Hydrocarbon Hydroxylases
7-Alkoxycoumarin O-Dealkylase
Mixed Function Oxygenases
Liver
Enzymes
Cytochrome P-450 Enzyme System
Lung
Experimental Diabetes Mellitus
Streptozocin
Intestines

Keywords

  • 7-Ethoxycoumarin-O-deethylase
  • Adrenalectomy
  • Aryl hydrocarbon hydroxylase
  • Cytochrome P-450
  • Diabetes
  • Extrahepatic
  • Hepatic

ASJC Scopus subject areas

  • Pharmacology

Cite this

Altered activities of hepatic and extrahepatic microsomal mixed function oxidase enzymes in diabetic and adrenalectomized diabetic rats. / Al-Turk, Walid A.; Stohs, Sidney J.; Roche, Edward B.

In: Pharmacology, Vol. 23, No. 6, 01.01.1981, p. 337-345.

Research output: Contribution to journalArticle

Al-Turk, Walid A. ; Stohs, Sidney J. ; Roche, Edward B. / Altered activities of hepatic and extrahepatic microsomal mixed function oxidase enzymes in diabetic and adrenalectomized diabetic rats. In: Pharmacology. 1981 ; Vol. 23, No. 6. pp. 337-345.
@article{12007f6863044a14ac85d70009ca1a9f,
title = "Altered activities of hepatic and extrahepatic microsomal mixed function oxidase enzymes in diabetic and adrenalectomized diabetic rats",
abstract = "Microsomal aryl hydrocarbon hydroxylase (AHH) and 7-ethoxycoumarin-O-deethylase (ECD) activities were determined in liver, lung and intestine of control, streptozotocin(STZ)-diabetic, and diabetic-adrenalectomized male and female rats. Hepatic cytochrome P-450 content was also determined. The diabetic state reduced hepatic AHH activity and increased ECD activity in control male rats, and failed to do so in the adrenalectomized male rats. The diabetic state increased hepatic AHH and ECD activities in the control female rats, while in the adrenalectomized female rats the activities of both enzymes were decreased compared to the control diabetic rats. STZ-induced diabetes produced a decrease in pulmonary AHH and ECD activities and increased intestinal AHH and ECD activities in both sexes. The diabetic state in the adrenalectomized rats resulted in further reduction in pulmonary AHH and ECD activities in both sexes, but failed to increase the intestinal AHH activity only in the female rat. Hepatic cytochrome P-450 contents were increased in the female but not male adrenalectomized rats when treated with STZ. The effects of STZ-induced diabetes in adrenalectomized rats on AHH. ECD and hepatic cytochrome P-450 content in liver, lung and intestine depended upon sex of animal, substrate and tissue.",
keywords = "7-Ethoxycoumarin-O-deethylase, Adrenalectomy, Aryl hydrocarbon hydroxylase, Cytochrome P-450, Diabetes, Extrahepatic, Hepatic",
author = "Al-Turk, {Walid A.} and Stohs, {Sidney J.} and Roche, {Edward B.}",
year = "1981",
month = "1",
day = "1",
doi = "10.1159/000137569",
language = "English (US)",
volume = "23",
pages = "337--345",
journal = "Pharmacology",
issn = "0031-7012",
publisher = "S. Karger AG",
number = "6",

}

TY - JOUR

T1 - Altered activities of hepatic and extrahepatic microsomal mixed function oxidase enzymes in diabetic and adrenalectomized diabetic rats

AU - Al-Turk, Walid A.

AU - Stohs, Sidney J.

AU - Roche, Edward B.

PY - 1981/1/1

Y1 - 1981/1/1

N2 - Microsomal aryl hydrocarbon hydroxylase (AHH) and 7-ethoxycoumarin-O-deethylase (ECD) activities were determined in liver, lung and intestine of control, streptozotocin(STZ)-diabetic, and diabetic-adrenalectomized male and female rats. Hepatic cytochrome P-450 content was also determined. The diabetic state reduced hepatic AHH activity and increased ECD activity in control male rats, and failed to do so in the adrenalectomized male rats. The diabetic state increased hepatic AHH and ECD activities in the control female rats, while in the adrenalectomized female rats the activities of both enzymes were decreased compared to the control diabetic rats. STZ-induced diabetes produced a decrease in pulmonary AHH and ECD activities and increased intestinal AHH and ECD activities in both sexes. The diabetic state in the adrenalectomized rats resulted in further reduction in pulmonary AHH and ECD activities in both sexes, but failed to increase the intestinal AHH activity only in the female rat. Hepatic cytochrome P-450 contents were increased in the female but not male adrenalectomized rats when treated with STZ. The effects of STZ-induced diabetes in adrenalectomized rats on AHH. ECD and hepatic cytochrome P-450 content in liver, lung and intestine depended upon sex of animal, substrate and tissue.

AB - Microsomal aryl hydrocarbon hydroxylase (AHH) and 7-ethoxycoumarin-O-deethylase (ECD) activities were determined in liver, lung and intestine of control, streptozotocin(STZ)-diabetic, and diabetic-adrenalectomized male and female rats. Hepatic cytochrome P-450 content was also determined. The diabetic state reduced hepatic AHH activity and increased ECD activity in control male rats, and failed to do so in the adrenalectomized male rats. The diabetic state increased hepatic AHH and ECD activities in the control female rats, while in the adrenalectomized female rats the activities of both enzymes were decreased compared to the control diabetic rats. STZ-induced diabetes produced a decrease in pulmonary AHH and ECD activities and increased intestinal AHH and ECD activities in both sexes. The diabetic state in the adrenalectomized rats resulted in further reduction in pulmonary AHH and ECD activities in both sexes, but failed to increase the intestinal AHH activity only in the female rat. Hepatic cytochrome P-450 contents were increased in the female but not male adrenalectomized rats when treated with STZ. The effects of STZ-induced diabetes in adrenalectomized rats on AHH. ECD and hepatic cytochrome P-450 content in liver, lung and intestine depended upon sex of animal, substrate and tissue.

KW - 7-Ethoxycoumarin-O-deethylase

KW - Adrenalectomy

KW - Aryl hydrocarbon hydroxylase

KW - Cytochrome P-450

KW - Diabetes

KW - Extrahepatic

KW - Hepatic

UR - http://www.scopus.com/inward/record.url?scp=0019800240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019800240&partnerID=8YFLogxK

U2 - 10.1159/000137569

DO - 10.1159/000137569

M3 - Article

C2 - 6975948

AN - SCOPUS:0019800240

VL - 23

SP - 337

EP - 345

JO - Pharmacology

JF - Pharmacology

SN - 0031-7012

IS - 6

ER -