Alterations of the biological characteristics of a colon carcinoma cell line by colon-derived substrata material

D. Boyd, G. Florent, S. Chakrabarty, D. Brattain, M. G. Brattain

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

This study documents the ability of substrata material derived from well but not poorly differentiated colon carcinoma cells to alter the biological characteristics of a separate colon carcinoma cell line (MOSER(SF)). To assess changes induced by the presence of these substrata, MOSER(SF) cells were screened for (a) morphological features, (b) secretion of carcinoembryonic antigen (CEA), (c) alteration of urokinase levels, and (d) sensitivity to the growth-inhibitory peptide transforming growth factor β. Morphologically, MOSER(SF) cells grown on plastic displayed a rounded shape and could be detached by agitation. Subculturing of these cells onto substrata laid down by well differentiated (mature) colon carcinoma cells resulted in cell attachment and spreading. These changes did not manifest themselves when cells were plated on material derived from poorly differentiated (primitive) colon cells. Conditioned medium from MOSER(SF) cells grown on plastic or on colon-derived material from the well and poorly differentiated colon cells were compared for CEA levels. Substrata derived from undifferentiated cells were without effect on assayable CEA (substrata absent, 1.4 ng/ml/106 cells/72 h; substrata present, 1.4-1.7 ng/ml/106 cells/72 h). However, growth of MOSER(SF) cells on material deposited by well differentiated colon cells resulted in a 3-fold increase in the level of CEA. Spent medium was also analyzed for urokinase. A high level of the protease (20.3 ng/ml/106 cells/72 h) was expressed by MOSER(SF) cells. The concentration of the enzyme was reduced by over 50% when MOSER(SF) cells were propagated on substrata laid down by well differentiated cells. An enhanced sensitivity to the growth-retarding effects of transforming growth factor β was seen with certain substrata. On plastic, transforming growth factor β inhibited proliferation of MOSER(SF) cells with a median effective concentration of 0.65 ng/ml. However, on substrata from mature but not primitive cells, MOSER(SF) cells exhibited an increased sensitivity to the peptide (median effective concentration, 0.16 ng/ml). Colon-derived material obtained from both well differentiated and poorly differentiated colon carcinoma cells was compared after [35S]methionine metabolic labeling. More [35S]methionine was incorporated into the material from the 'mature' colon cells. The substrata could also be distinguished by quantitative differences in a number of high molecular weight proteins. Immunofluorescence of colon-deposited material revealed the presence of laminin and fibronectin.

Original languageEnglish (US)
Pages (from-to)2825-2831
Number of pages7
JournalCancer Research
Volume48
Issue number10
StatePublished - 1988
Externally publishedYes

Fingerprint

Colon
Carcinoma
Cell Line
Carcinoembryonic Antigen
Transforming Growth Factors
Plastics
Urokinase-Type Plasminogen Activator
Methionine
Growth
Peptides
Laminin
Conditioned Culture Medium
Fibronectins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Boyd, D., Florent, G., Chakrabarty, S., Brattain, D., & Brattain, M. G. (1988). Alterations of the biological characteristics of a colon carcinoma cell line by colon-derived substrata material. Cancer Research, 48(10), 2825-2831.

Alterations of the biological characteristics of a colon carcinoma cell line by colon-derived substrata material. / Boyd, D.; Florent, G.; Chakrabarty, S.; Brattain, D.; Brattain, M. G.

In: Cancer Research, Vol. 48, No. 10, 1988, p. 2825-2831.

Research output: Contribution to journalArticle

Boyd, D, Florent, G, Chakrabarty, S, Brattain, D & Brattain, MG 1988, 'Alterations of the biological characteristics of a colon carcinoma cell line by colon-derived substrata material', Cancer Research, vol. 48, no. 10, pp. 2825-2831.
Boyd D, Florent G, Chakrabarty S, Brattain D, Brattain MG. Alterations of the biological characteristics of a colon carcinoma cell line by colon-derived substrata material. Cancer Research. 1988;48(10):2825-2831.
Boyd, D. ; Florent, G. ; Chakrabarty, S. ; Brattain, D. ; Brattain, M. G. / Alterations of the biological characteristics of a colon carcinoma cell line by colon-derived substrata material. In: Cancer Research. 1988 ; Vol. 48, No. 10. pp. 2825-2831.
@article{624d7ea565c94f25b9674c07908235bd,
title = "Alterations of the biological characteristics of a colon carcinoma cell line by colon-derived substrata material",
abstract = "This study documents the ability of substrata material derived from well but not poorly differentiated colon carcinoma cells to alter the biological characteristics of a separate colon carcinoma cell line (MOSER(SF)). To assess changes induced by the presence of these substrata, MOSER(SF) cells were screened for (a) morphological features, (b) secretion of carcinoembryonic antigen (CEA), (c) alteration of urokinase levels, and (d) sensitivity to the growth-inhibitory peptide transforming growth factor β. Morphologically, MOSER(SF) cells grown on plastic displayed a rounded shape and could be detached by agitation. Subculturing of these cells onto substrata laid down by well differentiated (mature) colon carcinoma cells resulted in cell attachment and spreading. These changes did not manifest themselves when cells were plated on material derived from poorly differentiated (primitive) colon cells. Conditioned medium from MOSER(SF) cells grown on plastic or on colon-derived material from the well and poorly differentiated colon cells were compared for CEA levels. Substrata derived from undifferentiated cells were without effect on assayable CEA (substrata absent, 1.4 ng/ml/106 cells/72 h; substrata present, 1.4-1.7 ng/ml/106 cells/72 h). However, growth of MOSER(SF) cells on material deposited by well differentiated colon cells resulted in a 3-fold increase in the level of CEA. Spent medium was also analyzed for urokinase. A high level of the protease (20.3 ng/ml/106 cells/72 h) was expressed by MOSER(SF) cells. The concentration of the enzyme was reduced by over 50{\%} when MOSER(SF) cells were propagated on substrata laid down by well differentiated cells. An enhanced sensitivity to the growth-retarding effects of transforming growth factor β was seen with certain substrata. On plastic, transforming growth factor β inhibited proliferation of MOSER(SF) cells with a median effective concentration of 0.65 ng/ml. However, on substrata from mature but not primitive cells, MOSER(SF) cells exhibited an increased sensitivity to the peptide (median effective concentration, 0.16 ng/ml). Colon-derived material obtained from both well differentiated and poorly differentiated colon carcinoma cells was compared after [35S]methionine metabolic labeling. More [35S]methionine was incorporated into the material from the 'mature' colon cells. The substrata could also be distinguished by quantitative differences in a number of high molecular weight proteins. Immunofluorescence of colon-deposited material revealed the presence of laminin and fibronectin.",
author = "D. Boyd and G. Florent and S. Chakrabarty and D. Brattain and Brattain, {M. G.}",
year = "1988",
language = "English (US)",
volume = "48",
pages = "2825--2831",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "10",

}

TY - JOUR

T1 - Alterations of the biological characteristics of a colon carcinoma cell line by colon-derived substrata material

AU - Boyd, D.

AU - Florent, G.

AU - Chakrabarty, S.

AU - Brattain, D.

AU - Brattain, M. G.

PY - 1988

Y1 - 1988

N2 - This study documents the ability of substrata material derived from well but not poorly differentiated colon carcinoma cells to alter the biological characteristics of a separate colon carcinoma cell line (MOSER(SF)). To assess changes induced by the presence of these substrata, MOSER(SF) cells were screened for (a) morphological features, (b) secretion of carcinoembryonic antigen (CEA), (c) alteration of urokinase levels, and (d) sensitivity to the growth-inhibitory peptide transforming growth factor β. Morphologically, MOSER(SF) cells grown on plastic displayed a rounded shape and could be detached by agitation. Subculturing of these cells onto substrata laid down by well differentiated (mature) colon carcinoma cells resulted in cell attachment and spreading. These changes did not manifest themselves when cells were plated on material derived from poorly differentiated (primitive) colon cells. Conditioned medium from MOSER(SF) cells grown on plastic or on colon-derived material from the well and poorly differentiated colon cells were compared for CEA levels. Substrata derived from undifferentiated cells were without effect on assayable CEA (substrata absent, 1.4 ng/ml/106 cells/72 h; substrata present, 1.4-1.7 ng/ml/106 cells/72 h). However, growth of MOSER(SF) cells on material deposited by well differentiated colon cells resulted in a 3-fold increase in the level of CEA. Spent medium was also analyzed for urokinase. A high level of the protease (20.3 ng/ml/106 cells/72 h) was expressed by MOSER(SF) cells. The concentration of the enzyme was reduced by over 50% when MOSER(SF) cells were propagated on substrata laid down by well differentiated cells. An enhanced sensitivity to the growth-retarding effects of transforming growth factor β was seen with certain substrata. On plastic, transforming growth factor β inhibited proliferation of MOSER(SF) cells with a median effective concentration of 0.65 ng/ml. However, on substrata from mature but not primitive cells, MOSER(SF) cells exhibited an increased sensitivity to the peptide (median effective concentration, 0.16 ng/ml). Colon-derived material obtained from both well differentiated and poorly differentiated colon carcinoma cells was compared after [35S]methionine metabolic labeling. More [35S]methionine was incorporated into the material from the 'mature' colon cells. The substrata could also be distinguished by quantitative differences in a number of high molecular weight proteins. Immunofluorescence of colon-deposited material revealed the presence of laminin and fibronectin.

AB - This study documents the ability of substrata material derived from well but not poorly differentiated colon carcinoma cells to alter the biological characteristics of a separate colon carcinoma cell line (MOSER(SF)). To assess changes induced by the presence of these substrata, MOSER(SF) cells were screened for (a) morphological features, (b) secretion of carcinoembryonic antigen (CEA), (c) alteration of urokinase levels, and (d) sensitivity to the growth-inhibitory peptide transforming growth factor β. Morphologically, MOSER(SF) cells grown on plastic displayed a rounded shape and could be detached by agitation. Subculturing of these cells onto substrata laid down by well differentiated (mature) colon carcinoma cells resulted in cell attachment and spreading. These changes did not manifest themselves when cells were plated on material derived from poorly differentiated (primitive) colon cells. Conditioned medium from MOSER(SF) cells grown on plastic or on colon-derived material from the well and poorly differentiated colon cells were compared for CEA levels. Substrata derived from undifferentiated cells were without effect on assayable CEA (substrata absent, 1.4 ng/ml/106 cells/72 h; substrata present, 1.4-1.7 ng/ml/106 cells/72 h). However, growth of MOSER(SF) cells on material deposited by well differentiated colon cells resulted in a 3-fold increase in the level of CEA. Spent medium was also analyzed for urokinase. A high level of the protease (20.3 ng/ml/106 cells/72 h) was expressed by MOSER(SF) cells. The concentration of the enzyme was reduced by over 50% when MOSER(SF) cells were propagated on substrata laid down by well differentiated cells. An enhanced sensitivity to the growth-retarding effects of transforming growth factor β was seen with certain substrata. On plastic, transforming growth factor β inhibited proliferation of MOSER(SF) cells with a median effective concentration of 0.65 ng/ml. However, on substrata from mature but not primitive cells, MOSER(SF) cells exhibited an increased sensitivity to the peptide (median effective concentration, 0.16 ng/ml). Colon-derived material obtained from both well differentiated and poorly differentiated colon carcinoma cells was compared after [35S]methionine metabolic labeling. More [35S]methionine was incorporated into the material from the 'mature' colon cells. The substrata could also be distinguished by quantitative differences in a number of high molecular weight proteins. Immunofluorescence of colon-deposited material revealed the presence of laminin and fibronectin.

UR - http://www.scopus.com/inward/record.url?scp=0023913731&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023913731&partnerID=8YFLogxK

M3 - Article

VL - 48

SP - 2825

EP - 2831

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 10

ER -