Alteration of the pig intestinal microbiome when vaccinated against or inoculated with porcine circovirus 2 using a multivariate analysis model

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Abstract

This study assessed the effect of porcine circovirus 2 (PCV2) vaccination compared with inoculation on the intestinal microbiome of piglets. Ninety-six weaned barrows (age 27 to 40 d and 7.1 kg BW) were either vaccinated for PCV2 (VAC) or inoculated with PCV2 (PCV) on d 0. Fecal samples were collected at d 0, 14, and 28 (n = 6/treatment). Microbial community structure was analyzed using Ion Torrent technology by sequencing the 16S rRNA gene. No differences (P > 0.10) were detected at the genus level between d 0 samples. Prevotella spp. decreased from d 14 to 28 in VAC pigs (P < 0.05) and was reduced compared with PCV at d 28 (P < 0.10). A core set of 383 operational taxonomic units (OTU), present in >80% of samples, were analyzed using a multivariate analysis by linear models. A total of 58 OTU were correlated with at least 1 phenotypic trait or fixed effect, that is, BW, day, or PCV status (P < 0.05, q < 0.05). Of these, 15 OTU were determined to be of the genus Prevotella. Abundances of Prevotella stercorea (r = 0.21) and Solitalea koreensis (r = 0.41) were shown to be associated with serum IgG concentration, whereas Intestinimonas butyriciproducens (r = –0.07) and Oscillibacter valericigenes (r = –0.09) were inversely associated with serum IgM concentration. Presence of P. stercorea (r = –0.11) was negatively associated with VAC pigs by d 28, whereas Ruminiclostridium ther-mosuccinogenes (r = 0.11) presence increased. These data suggest that Prevotella spp. flourish during a PCV challenge, irrespective of PCV status (vaccinated or inoculated), but decreased sooner in VAC pigs than PCV pigs. However, an age-dependent shift in the microbiome may also play a role.

Original languageEnglish (US)
Pages (from-to)387-390
Number of pages4
JournalJournal of animal science
Volume94
Issue number7
DOIs
StatePublished - Sep 2016

Fingerprint

Circovirus
Prevotella
Porcine circovirus-2
multivariate analysis
Swine
Multivariate Analysis
swine
vaccination
Microbiota
barrows
Serum
rRNA Genes
sampling
Immunoglobulin M
microbial communities
piglets
Linear Models
Vaccination
community structure
Immunoglobulin G

Keywords

  • Microbiome
  • Pig
  • Porcine circovirus 2
  • Vaccination

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

@article{349e188a375a497aa8e9cab2369ddda2,
title = "Alteration of the pig intestinal microbiome when vaccinated against or inoculated with porcine circovirus 2 using a multivariate analysis model",
abstract = "This study assessed the effect of porcine circovirus 2 (PCV2) vaccination compared with inoculation on the intestinal microbiome of piglets. Ninety-six weaned barrows (age 27 to 40 d and 7.1 kg BW) were either vaccinated for PCV2 (VAC) or inoculated with PCV2 (PCV) on d 0. Fecal samples were collected at d 0, 14, and 28 (n = 6/treatment). Microbial community structure was analyzed using Ion Torrent technology by sequencing the 16S rRNA gene. No differences (P > 0.10) were detected at the genus level between d 0 samples. Prevotella spp. decreased from d 14 to 28 in VAC pigs (P < 0.05) and was reduced compared with PCV at d 28 (P < 0.10). A core set of 383 operational taxonomic units (OTU), present in >80{\%} of samples, were analyzed using a multivariate analysis by linear models. A total of 58 OTU were correlated with at least 1 phenotypic trait or fixed effect, that is, BW, day, or PCV status (P < 0.05, q < 0.05). Of these, 15 OTU were determined to be of the genus Prevotella. Abundances of Prevotella stercorea (r = 0.21) and Solitalea koreensis (r = 0.41) were shown to be associated with serum IgG concentration, whereas Intestinimonas butyriciproducens (r = –0.07) and Oscillibacter valericigenes (r = –0.09) were inversely associated with serum IgM concentration. Presence of P. stercorea (r = –0.11) was negatively associated with VAC pigs by d 28, whereas Ruminiclostridium ther-mosuccinogenes (r = 0.11) presence increased. These data suggest that Prevotella spp. flourish during a PCV challenge, irrespective of PCV status (vaccinated or inoculated), but decreased sooner in VAC pigs than PCV pigs. However, an age-dependent shift in the microbiome may also play a role.",
keywords = "Microbiome, Pig, Porcine circovirus 2, Vaccination",
author = "{van Sambeek}, {D. M.} and H. Tran and Fernando, {Samodha C} and Ciobanu, {Daniel C} and Miller, {Phillip S} and Burkey, {Thomas E}",
year = "2016",
month = "9",
doi = "10.2527/jas2015-9861",
language = "English (US)",
volume = "94",
pages = "387--390",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "American Society of Animal Science",
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T1 - Alteration of the pig intestinal microbiome when vaccinated against or inoculated with porcine circovirus 2 using a multivariate analysis model

AU - van Sambeek, D. M.

AU - Tran, H.

AU - Fernando, Samodha C

AU - Ciobanu, Daniel C

AU - Miller, Phillip S

AU - Burkey, Thomas E

PY - 2016/9

Y1 - 2016/9

N2 - This study assessed the effect of porcine circovirus 2 (PCV2) vaccination compared with inoculation on the intestinal microbiome of piglets. Ninety-six weaned barrows (age 27 to 40 d and 7.1 kg BW) were either vaccinated for PCV2 (VAC) or inoculated with PCV2 (PCV) on d 0. Fecal samples were collected at d 0, 14, and 28 (n = 6/treatment). Microbial community structure was analyzed using Ion Torrent technology by sequencing the 16S rRNA gene. No differences (P > 0.10) were detected at the genus level between d 0 samples. Prevotella spp. decreased from d 14 to 28 in VAC pigs (P < 0.05) and was reduced compared with PCV at d 28 (P < 0.10). A core set of 383 operational taxonomic units (OTU), present in >80% of samples, were analyzed using a multivariate analysis by linear models. A total of 58 OTU were correlated with at least 1 phenotypic trait or fixed effect, that is, BW, day, or PCV status (P < 0.05, q < 0.05). Of these, 15 OTU were determined to be of the genus Prevotella. Abundances of Prevotella stercorea (r = 0.21) and Solitalea koreensis (r = 0.41) were shown to be associated with serum IgG concentration, whereas Intestinimonas butyriciproducens (r = –0.07) and Oscillibacter valericigenes (r = –0.09) were inversely associated with serum IgM concentration. Presence of P. stercorea (r = –0.11) was negatively associated with VAC pigs by d 28, whereas Ruminiclostridium ther-mosuccinogenes (r = 0.11) presence increased. These data suggest that Prevotella spp. flourish during a PCV challenge, irrespective of PCV status (vaccinated or inoculated), but decreased sooner in VAC pigs than PCV pigs. However, an age-dependent shift in the microbiome may also play a role.

AB - This study assessed the effect of porcine circovirus 2 (PCV2) vaccination compared with inoculation on the intestinal microbiome of piglets. Ninety-six weaned barrows (age 27 to 40 d and 7.1 kg BW) were either vaccinated for PCV2 (VAC) or inoculated with PCV2 (PCV) on d 0. Fecal samples were collected at d 0, 14, and 28 (n = 6/treatment). Microbial community structure was analyzed using Ion Torrent technology by sequencing the 16S rRNA gene. No differences (P > 0.10) were detected at the genus level between d 0 samples. Prevotella spp. decreased from d 14 to 28 in VAC pigs (P < 0.05) and was reduced compared with PCV at d 28 (P < 0.10). A core set of 383 operational taxonomic units (OTU), present in >80% of samples, were analyzed using a multivariate analysis by linear models. A total of 58 OTU were correlated with at least 1 phenotypic trait or fixed effect, that is, BW, day, or PCV status (P < 0.05, q < 0.05). Of these, 15 OTU were determined to be of the genus Prevotella. Abundances of Prevotella stercorea (r = 0.21) and Solitalea koreensis (r = 0.41) were shown to be associated with serum IgG concentration, whereas Intestinimonas butyriciproducens (r = –0.07) and Oscillibacter valericigenes (r = –0.09) were inversely associated with serum IgM concentration. Presence of P. stercorea (r = –0.11) was negatively associated with VAC pigs by d 28, whereas Ruminiclostridium ther-mosuccinogenes (r = 0.11) presence increased. These data suggest that Prevotella spp. flourish during a PCV challenge, irrespective of PCV status (vaccinated or inoculated), but decreased sooner in VAC pigs than PCV pigs. However, an age-dependent shift in the microbiome may also play a role.

KW - Microbiome

KW - Pig

KW - Porcine circovirus 2

KW - Vaccination

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DO - 10.2527/jas2015-9861

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