Alteration in the behavior of a colon carcinoma cell line by extracellular matrix components

Douglas Boyd, Germaine Florent, Karla Childress-Fields, Michael G. Brattain

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

It was previously demonstrated that substrata derived from well differentiated colon carcinoma cell lines induced a more benign program in a separate malignant colon cell line, MOSERaf. This study attempts to define a role for extracellular matrix components in the biological events of MOSERaf cells. Alterations in morphology, secreted carcinoembryonic antigen (CEA) and urokinase brought about by individual components were determined. Laminin induced similar changes to colon-derived substrata in that there was increased cell attachment and spreading, a 4-fold elevation in CEA and a 45% reduction in urokinase. Fibronectin stimulated cell attachment without altered morphology and reduced the amount of plasminogen activator. CEA values, however, remained unchanged. Growth of MOSERaf cells on all types of collagen failed to elicit any change in cell shape or CEA. However, type I III collagen raised urokinase levels by 40%. Transforming growth factor β (TGF-β) induces cellular laminin and fibronectin, promotes cell attachment, and spreading, elevates CEA and diminishes urokinase. These data argue for a role for laminin and possibly fibronectin in the governing of biological events culminating in a more mature colon cell.

Original languageEnglish (US)
Pages (from-to)81-90
Number of pages10
JournalCancer Letters
Volume41
Issue number1
DOIs
StatePublished - Jul 1988

Fingerprint

Carcinoembryonic Antigen
Extracellular Matrix
Colon
Urokinase-Type Plasminogen Activator
Carcinoma
Cell Line
Laminin
Fibronectins
Collagen Type III
Cell Shape
Plasminogen Activators
Transforming Growth Factors
Collagen Type I
Collagen
Growth

Keywords

  • Carcinoembryonic antigen
  • Colon carcinoma cell line
  • Extracellular matrix components
  • Laminin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Alteration in the behavior of a colon carcinoma cell line by extracellular matrix components. / Boyd, Douglas; Florent, Germaine; Childress-Fields, Karla; Brattain, Michael G.

In: Cancer Letters, Vol. 41, No. 1, 07.1988, p. 81-90.

Research output: Contribution to journalArticle

Boyd, Douglas ; Florent, Germaine ; Childress-Fields, Karla ; Brattain, Michael G. / Alteration in the behavior of a colon carcinoma cell line by extracellular matrix components. In: Cancer Letters. 1988 ; Vol. 41, No. 1. pp. 81-90.
@article{ab5bcc22f7014e48bce1f6bed8bf180a,
title = "Alteration in the behavior of a colon carcinoma cell line by extracellular matrix components",
abstract = "It was previously demonstrated that substrata derived from well differentiated colon carcinoma cell lines induced a more benign program in a separate malignant colon cell line, MOSERaf. This study attempts to define a role for extracellular matrix components in the biological events of MOSERaf cells. Alterations in morphology, secreted carcinoembryonic antigen (CEA) and urokinase brought about by individual components were determined. Laminin induced similar changes to colon-derived substrata in that there was increased cell attachment and spreading, a 4-fold elevation in CEA and a 45{\%} reduction in urokinase. Fibronectin stimulated cell attachment without altered morphology and reduced the amount of plasminogen activator. CEA values, however, remained unchanged. Growth of MOSERaf cells on all types of collagen failed to elicit any change in cell shape or CEA. However, type I III collagen raised urokinase levels by 40{\%}. Transforming growth factor β (TGF-β) induces cellular laminin and fibronectin, promotes cell attachment, and spreading, elevates CEA and diminishes urokinase. These data argue for a role for laminin and possibly fibronectin in the governing of biological events culminating in a more mature colon cell.",
keywords = "Carcinoembryonic antigen, Colon carcinoma cell line, Extracellular matrix components, Laminin",
author = "Douglas Boyd and Germaine Florent and Karla Childress-Fields and Brattain, {Michael G.}",
year = "1988",
month = "7",
doi = "10.1016/0304-3835(88)90058-4",
language = "English (US)",
volume = "41",
pages = "81--90",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Alteration in the behavior of a colon carcinoma cell line by extracellular matrix components

AU - Boyd, Douglas

AU - Florent, Germaine

AU - Childress-Fields, Karla

AU - Brattain, Michael G.

PY - 1988/7

Y1 - 1988/7

N2 - It was previously demonstrated that substrata derived from well differentiated colon carcinoma cell lines induced a more benign program in a separate malignant colon cell line, MOSERaf. This study attempts to define a role for extracellular matrix components in the biological events of MOSERaf cells. Alterations in morphology, secreted carcinoembryonic antigen (CEA) and urokinase brought about by individual components were determined. Laminin induced similar changes to colon-derived substrata in that there was increased cell attachment and spreading, a 4-fold elevation in CEA and a 45% reduction in urokinase. Fibronectin stimulated cell attachment without altered morphology and reduced the amount of plasminogen activator. CEA values, however, remained unchanged. Growth of MOSERaf cells on all types of collagen failed to elicit any change in cell shape or CEA. However, type I III collagen raised urokinase levels by 40%. Transforming growth factor β (TGF-β) induces cellular laminin and fibronectin, promotes cell attachment, and spreading, elevates CEA and diminishes urokinase. These data argue for a role for laminin and possibly fibronectin in the governing of biological events culminating in a more mature colon cell.

AB - It was previously demonstrated that substrata derived from well differentiated colon carcinoma cell lines induced a more benign program in a separate malignant colon cell line, MOSERaf. This study attempts to define a role for extracellular matrix components in the biological events of MOSERaf cells. Alterations in morphology, secreted carcinoembryonic antigen (CEA) and urokinase brought about by individual components were determined. Laminin induced similar changes to colon-derived substrata in that there was increased cell attachment and spreading, a 4-fold elevation in CEA and a 45% reduction in urokinase. Fibronectin stimulated cell attachment without altered morphology and reduced the amount of plasminogen activator. CEA values, however, remained unchanged. Growth of MOSERaf cells on all types of collagen failed to elicit any change in cell shape or CEA. However, type I III collagen raised urokinase levels by 40%. Transforming growth factor β (TGF-β) induces cellular laminin and fibronectin, promotes cell attachment, and spreading, elevates CEA and diminishes urokinase. These data argue for a role for laminin and possibly fibronectin in the governing of biological events culminating in a more mature colon cell.

KW - Carcinoembryonic antigen

KW - Colon carcinoma cell line

KW - Extracellular matrix components

KW - Laminin

UR - http://www.scopus.com/inward/record.url?scp=0023944662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023944662&partnerID=8YFLogxK

U2 - 10.1016/0304-3835(88)90058-4

DO - 10.1016/0304-3835(88)90058-4

M3 - Article

C2 - 3164644

AN - SCOPUS:0023944662

VL - 41

SP - 81

EP - 90

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -