Alpha thalassemia/mental retardation syndrome X-linked gene product ATRX is required for proper replication restart and cellular resistance to replication stress

Justin Wai Chung Leung, Gargi Ghosal, Wenqi Wang, Xi Shen, Jiadong Wang, Lei Li, Junjie Chen

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Alpha thalassemia/mental retardation syndrome X-linked (ATRX) is a member of the SWI/SNF protein family of DNA-dependent ATPases. It functions as a chromatin remodeler and is classified as an SNF2-like helicase. Here, we showed somatic knock-out of ATRX displayed perturbed S-phase progression as well as hypersensitivity to replication stress. ATRX is recruited to sites of DNA damage, required for efficient checkpoint activation and faithful replication restart. In addition, we identified ATRX as a binding partner of MRE11-RAD50-NBS1 (MRN) complex. Together, these results suggest a non-canonical function of ATRX in guarding genomic stability.

Original languageEnglish (US)
Pages (from-to)6342-6350
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number9
DOIs
StatePublished - Mar 1 2013
Externally publishedYes

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alpha-Thalassemia
X-Linked Genes
Intellectual Disability
Chromatin
Adenosine Triphosphatases
Genes
Chemical activation
DNA
Proteins
Genomic Instability
S Phase
DNA Damage
Hypersensitivity
ATR-X syndrome

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Alpha thalassemia/mental retardation syndrome X-linked gene product ATRX is required for proper replication restart and cellular resistance to replication stress. / Leung, Justin Wai Chung; Ghosal, Gargi; Wang, Wenqi; Shen, Xi; Wang, Jiadong; Li, Lei; Chen, Junjie.

In: Journal of Biological Chemistry, Vol. 288, No. 9, 01.03.2013, p. 6342-6350.

Research output: Contribution to journalArticle

Leung, Justin Wai Chung ; Ghosal, Gargi ; Wang, Wenqi ; Shen, Xi ; Wang, Jiadong ; Li, Lei ; Chen, Junjie. / Alpha thalassemia/mental retardation syndrome X-linked gene product ATRX is required for proper replication restart and cellular resistance to replication stress. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 9. pp. 6342-6350.
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AU - Wang, Jiadong

AU - Li, Lei

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