Alpha and beta adrenergic agonists stimulate water absorption in the rat proximal tubule

Edward J. Weinman, Steven C. Sansom, Thomas F. Knight, Harry O. Senekjian

Research output: Contribution to journalArticle

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Abstract

Simultaneous capillary and luminal microperfusion studies were performed in the rat proximal tubule to determine the effects of the beta agonist isoproterenol and the alpha agonist phenylephrine on water absorption. Capillary and luminal perfusion solutions were composed such that organic solutes were not present, no bicarbonate was present in the lumen, and no chloride gradient was imposed. Under such conditions, water absorption (Jv) averaged 0.36±0.11 nl·min-1·mm-1. The addition of isoproterenol to the capillary solution in concentrations of 10-6 and 10-4m resulted in significantly higher Jv's of 0.68±0.10 and 0.71±0.11 nl·min-1·mm-1, respectively. The enhancing effect of isoproterenol was inhibited by the beta blocker propranolol (10-4m), but not by the alpha blocker phentolamine (10-7m). The addition of phenylephrine (10-6m) to the capillary perfusion solution also resulted in a significantly higher Jv of 0.84±0.14 nl·min-1·mm-1, an effect inhibited by phentolamine (10-7m), but not by propranolol (10-4m). Neither phentolamine nor propranolol alone in the concentrations indicated had an effect on water absorption. These experiments indicate that both alpha and beta agonists stimulate water absorption in the superficial proximal tubule of the rat. This effect appears to be relatively specific for each class of agonist, as demonstrated by the effects of the specific antagonists.

Original languageEnglish (US)
Pages (from-to)107-111
Number of pages5
JournalThe Journal of Membrane Biology
Volume69
Issue number2
DOIs
StatePublished - Jun 1 1982

Fingerprint

Adrenergic alpha-Agonists
Adrenergic beta-Agonists
Phentolamine
Isoproterenol
Propranolol
Water
Phenylephrine
Perfusion
Bicarbonates
Chlorides

Keywords

  • catecholamines
  • microperfusion
  • sodium transport

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

Cite this

Alpha and beta adrenergic agonists stimulate water absorption in the rat proximal tubule. / Weinman, Edward J.; Sansom, Steven C.; Knight, Thomas F.; Senekjian, Harry O.

In: The Journal of Membrane Biology, Vol. 69, No. 2, 01.06.1982, p. 107-111.

Research output: Contribution to journalArticle

Weinman, Edward J. ; Sansom, Steven C. ; Knight, Thomas F. ; Senekjian, Harry O. / Alpha and beta adrenergic agonists stimulate water absorption in the rat proximal tubule. In: The Journal of Membrane Biology. 1982 ; Vol. 69, No. 2. pp. 107-111.
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