Allosteric modulation of the RNA polymerase catalytic reaction is an essential component of transcription control by rifamycins

Irina Artsimovitch, Marina N. Vassylyeva, Dmitri Svetlov, Vladimir Svetlov, Anna Perederina, Noriyuki Igarashi, Naohiro Matsugaki, Soichi Wakatsuki, Tahir H. Tahirov, Dmitry G. Vassylyev

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Rifamycins, the clinically important antibiotics, target bacterial RNA polymerase (RNAP). A proposed mechanism in which rifamycins sterically block the extension of nascent RNA beyond three nucleotides does not alone explain why certain RNAP mutations confer resistance to some but not other rifamycins. Here we show that unlike rifampicin and rifapentin, and contradictory to the steric model, rifabutin inhibits formation of the first and second phosphodiester bonds. We report 2.5 Å resolution structures of rifabutin and rifapentin complexed with the Thermus thermophilus RNAP holoenzyme. The structures reveal functionally important distinct interactions of antibiotics with the initiation σ factor. Strikingly, both complexes lack the catalytic Mg2+ ion observed in the apo-holoenzyme, whereas an increase in Mg2+ concentration confers resistance to rifamycins. We propose that a rifamycin-induced signal is transmitted over ∼19 Å to the RNAP active site to slow down catalysis. Based on structural predictions, we designed enzyme substitutions that apparently interrupt this allosteric signal.

Original languageEnglish (US)
Pages (from-to)351-363
Number of pages13
JournalCell
Volume122
Issue number3
DOIs
StatePublished - Aug 12 2005

Fingerprint

Rifamycins
DNA-Directed RNA Polymerases
Transcription
Modulation
Rifabutin
Holoenzymes
Bacterial RNA
Anti-Bacterial Agents
Thermus thermophilus
Peptide Initiation Factors
Rifampin
Catalysis
Catalytic Domain
Substitution reactions
Nucleotides
RNA
Ions
Mutation
Enzymes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Artsimovitch, I., Vassylyeva, M. N., Svetlov, D., Svetlov, V., Perederina, A., Igarashi, N., ... Vassylyev, D. G. (2005). Allosteric modulation of the RNA polymerase catalytic reaction is an essential component of transcription control by rifamycins. Cell, 122(3), 351-363. https://doi.org/10.1016/j.cell.2005.07.014

Allosteric modulation of the RNA polymerase catalytic reaction is an essential component of transcription control by rifamycins. / Artsimovitch, Irina; Vassylyeva, Marina N.; Svetlov, Dmitri; Svetlov, Vladimir; Perederina, Anna; Igarashi, Noriyuki; Matsugaki, Naohiro; Wakatsuki, Soichi; Tahirov, Tahir H.; Vassylyev, Dmitry G.

In: Cell, Vol. 122, No. 3, 12.08.2005, p. 351-363.

Research output: Contribution to journalArticle

Artsimovitch, I, Vassylyeva, MN, Svetlov, D, Svetlov, V, Perederina, A, Igarashi, N, Matsugaki, N, Wakatsuki, S, Tahirov, TH & Vassylyev, DG 2005, 'Allosteric modulation of the RNA polymerase catalytic reaction is an essential component of transcription control by rifamycins', Cell, vol. 122, no. 3, pp. 351-363. https://doi.org/10.1016/j.cell.2005.07.014
Artsimovitch I, Vassylyeva MN, Svetlov D, Svetlov V, Perederina A, Igarashi N et al. Allosteric modulation of the RNA polymerase catalytic reaction is an essential component of transcription control by rifamycins. Cell. 2005 Aug 12;122(3):351-363. https://doi.org/10.1016/j.cell.2005.07.014
Artsimovitch, Irina ; Vassylyeva, Marina N. ; Svetlov, Dmitri ; Svetlov, Vladimir ; Perederina, Anna ; Igarashi, Noriyuki ; Matsugaki, Naohiro ; Wakatsuki, Soichi ; Tahirov, Tahir H. ; Vassylyev, Dmitry G. / Allosteric modulation of the RNA polymerase catalytic reaction is an essential component of transcription control by rifamycins. In: Cell. 2005 ; Vol. 122, No. 3. pp. 351-363.
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