Alloreactive and syngeneic CTL are comparably dependent on interaction with MHC class I α-helical residues

Tara M C Hornell, Joyce C Solheim, Nancy B. Myers, William E. Gillanders, Ganesaratnam K. Balendiran, Ted H. Hansen, Janet M. Connolly

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The molecular basis for the difference in the strength of T cell responses to self vs alloantigens is unknown, but may reflect how T cells are selected in the thymus. Because T cells with a high affinity for foreign as opposed to self MHC molecules are able to mature, it has been proposed that alloreactive T cells may be more strongly dependent upon interaction with MHC residues than are self-restricted T cells. This study was undertaken to rigorously address this hypothesis. Whereas other studies have compared self vs alloantigen recognition of different MHC alleles by a single T cell clone, we have compared self vs alloantigen recognition of a single MHC allele, H- 2L(d), by a large panel of self-restricted and alloreactive T cell clones. Target cells expressing L(d) molecules mutated at several different potential TCR contact residues were analyzed to determine which residues are important for recognition by self-restricted vs alloreactive T cells. We unequivocally demonstrate that self-restricted and alloreactive T cells do not differ, but rather are comparably dependent on interaction with MHC residues. Importantly, both self-restricted and alloreactive T cells are dependent upon the same MHC residues as primary contacts and, in addition, share a common recognition pattern of L(d). Furthermore, our analysis enables us to provide a model for allotype-specific T cell recognition of L(d) vs Kb class I molecules.

Original languageEnglish (US)
Pages (from-to)3217-3225
Number of pages9
JournalJournal of Immunology
Volume163
Issue number6
StatePublished - Sep 15 1999

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T-Lymphocytes
Isoantigens
Clone Cells
Alleles
Thymus Gland

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Hornell, T. M. C., Solheim, J. C., Myers, N. B., Gillanders, W. E., Balendiran, G. K., Hansen, T. H., & Connolly, J. M. (1999). Alloreactive and syngeneic CTL are comparably dependent on interaction with MHC class I α-helical residues. Journal of Immunology, 163(6), 3217-3225.

Alloreactive and syngeneic CTL are comparably dependent on interaction with MHC class I α-helical residues. / Hornell, Tara M C; Solheim, Joyce C; Myers, Nancy B.; Gillanders, William E.; Balendiran, Ganesaratnam K.; Hansen, Ted H.; Connolly, Janet M.

In: Journal of Immunology, Vol. 163, No. 6, 15.09.1999, p. 3217-3225.

Research output: Contribution to journalArticle

Hornell, TMC, Solheim, JC, Myers, NB, Gillanders, WE, Balendiran, GK, Hansen, TH & Connolly, JM 1999, 'Alloreactive and syngeneic CTL are comparably dependent on interaction with MHC class I α-helical residues', Journal of Immunology, vol. 163, no. 6, pp. 3217-3225.
Hornell TMC, Solheim JC, Myers NB, Gillanders WE, Balendiran GK, Hansen TH et al. Alloreactive and syngeneic CTL are comparably dependent on interaction with MHC class I α-helical residues. Journal of Immunology. 1999 Sep 15;163(6):3217-3225.
Hornell, Tara M C ; Solheim, Joyce C ; Myers, Nancy B. ; Gillanders, William E. ; Balendiran, Ganesaratnam K. ; Hansen, Ted H. ; Connolly, Janet M. / Alloreactive and syngeneic CTL are comparably dependent on interaction with MHC class I α-helical residues. In: Journal of Immunology. 1999 ; Vol. 163, No. 6. pp. 3217-3225.
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