Allogeneic transplantation of blood-derived, T cell-depleted hemopoietic stem cells after myeloablative treatment in a patient with acute lymphoblastic leukemia

A. Kessinger, D. M. Smith, S. E. Strandjord, J. D. Landmark, D. C. Dooley, P. Law, P. F. Coccia, Phyllis Irene Warkentin, D. D. Weisenburger, James Olen Armitage

Research output: Contribution to journalArticle

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Abstract

This report describes an allogeneic peripheral blood stem cell transplant in a patient who had received marrow ablative therapy. The patient was an 18-year-old white male with acute lymphocytic leukemia in third remission for whom an allogeneic bone marrow transplant was recommended. His HLA-identical sibling preferred to donate peripheral blood stem cells rather than marrow. The donor cells were collected with 10 apheresis procedures and depleted of T lymphocytes to prevent excessive graft-versus-host disease. Nine collections were cryopreserved. The patient received high-dose cytosine arabinoside and 12 Gy of total body irradiation, followed by infusion of all cryopreserved donor cells. A portion of the tenth apheresis product collected on the day of transplant containing 1.8 x 109 T lymphocytes was infused without further processing to approximate the number of T lymphocytes given in an allogeneic bone marrow transplant; the remainder was T lymphocyte depleted and infused. More than 1 x 109/l granulocytes were present on day +11. A bone marrow biopsy on day +27 showed trilineage engraftment. Cytogenetic studies demonstrated that the recipient's marrow and peripheral blood were populated exclusively with donor cells. Allogeneic peripheral stem cell transplantation produced an early hematopoietic engraftment. Since the patient died on day +32, sustained engraftment could not be evaluated.

Original languageEnglish (US)
Pages (from-to)643-646
Number of pages4
JournalBone marrow transplantation
Volume4
Issue number6
StatePublished - Dec 1 1989

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Homologous Transplantation
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Stem Cells
Bone Marrow
T-Lymphocytes
Transplants
Blood Component Removal
Tissue Donors
Therapeutics
Peripheral Blood Stem Cell Transplantation
Whole-Body Irradiation
Cytarabine
Graft vs Host Disease
Granulocytes
Cytogenetics
Siblings
Biopsy

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Allogeneic transplantation of blood-derived, T cell-depleted hemopoietic stem cells after myeloablative treatment in a patient with acute lymphoblastic leukemia. / Kessinger, A.; Smith, D. M.; Strandjord, S. E.; Landmark, J. D.; Dooley, D. C.; Law, P.; Coccia, P. F.; Warkentin, Phyllis Irene; Weisenburger, D. D.; Armitage, James Olen.

In: Bone marrow transplantation, Vol. 4, No. 6, 01.12.1989, p. 643-646.

Research output: Contribution to journalArticle

Kessinger, A. ; Smith, D. M. ; Strandjord, S. E. ; Landmark, J. D. ; Dooley, D. C. ; Law, P. ; Coccia, P. F. ; Warkentin, Phyllis Irene ; Weisenburger, D. D. ; Armitage, James Olen. / Allogeneic transplantation of blood-derived, T cell-depleted hemopoietic stem cells after myeloablative treatment in a patient with acute lymphoblastic leukemia. In: Bone marrow transplantation. 1989 ; Vol. 4, No. 6. pp. 643-646.
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