Abstract

We hypothesized that a high-speed all-digital video imaging system, with computerized analysis, would precisely capture and measure ciliary beat frequency (CBF) and would shorten the time from data capture to data analysis. We compared a conventional analog video system with a new high-speed digital system we developed for CBF analysis. Using ciliated primary bovine bronchial epithelial cells we made simultaneous analog and digital CBF measurements of the same region of interest (ROI) while temperature was varied. This yielded nearly identical data over a wide range of frequencies (7-15 Hz) using either system. Unlike the digital system however, the analog system did not accurately detect CBF above 15 Hz (temperatures higher than 30°C). We also compared ROI analysis with a new analysis algorithm we have named whole-field analysis (WFA). WFA measurement of CBF agreed with ROI and reduced operator time required to analyse data by more than 90% compared with the analog system. We conclude that all-digital computerized CBF analysis correlates closely with standard video methods, markedly speeds up data analysis and provides new ways, including WFA, to analyse entire fields of motile cilia simultaneously. We have termed this system 'Sisson-Ammons Video Analysis' (SAVA).

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalJournal of Microscopy
Volume211
Issue number2
DOIs
StatePublished - Aug 1 2003

Fingerprint

beat frequencies
analogs
digital systems
Temperature
Cilia
Imaging systems
Data acquisition
Epithelial Cells
high speed
frequency measurement
operators

Keywords

  • Airway epithelial cells
  • Ciliary beat frequency
  • Image analysis
  • Lung cilia measurement
  • SAVA
  • Temperature
  • Whole-field analysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

All-digital image capture and whole-field analysis of ciliary beat frequency. / Sisson, Joseph Harold; Stoner, J. A.; Ammons, B. A.; Wyatt, Todd A.

In: Journal of Microscopy, Vol. 211, No. 2, 01.08.2003, p. 103-111.

Research output: Contribution to journalArticle

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