Alcohol consumption is associated with alterations in macrophage responses to interferon-γ and infection by Salmonella typhimurium

Don A. Sibley, Natalia A Osna, Charles Kusynski, Linda Wilkie, Thomas R. Jerrells

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Abuse of ethanol (EtOH) by human beings and administration of EtOH to experimental animals has been shown to be associated with a suppression of the immune system. Consumption of EtOH has also been associated with an increased incidence and severity of infections of human beings and experimental animals, which has been attributed to the immunosuppression associated with EtOH consumption. It has been shown that EtOH also affects the function of macrophages (MØ), which are important effector cells in the innate and adaptive immune responses to infectious agents. The present studies were designed to investigate the effects of EtOH on MØ function with an animal model of EtOH consumption. The experiments reported in this paper were done with inflammatory MØ and were designed to determine the effects of EtOH on the ability of inflammatory MØ to respond to interferon-γ (IFN-γ) to control the intracellular growth of Salmonella typhimurium, as well as the production of proinflammatory cytokines and nitric oxide. The ability of MØ from EtOH-fed mice to respond to bacterial endotoxin (lipopolysaccharide (LPS)) and IFN-γ was also evaluated. MØ isolated from EtOH-fed mice did not respond as well to IFN-γ as MØ isolated from control mice as measured by control of S. typhimurium, as well as tumor necrosis factor (TNF) and nitric oxide production. Interleukin (IL)-6 production was not affected. Activation of MØ from EtOH-fed mice with LPS and IFN-γ produced levels of nitric oxide and TNF only slightly less than the levels seen in MØ from control mice, but a significant decrease in IL-6 was seen when MØ from EtOH-fed mice were stimulated with this combination. Flow cytometric analyses showed that IFN-γ receptor expression was not affected by EtOH. Together the data presented in this paper show that consumption of EtOH is associated with changes in inflammatory MØ responses to IFN-γ.

Original languageEnglish (US)
Pages (from-to)73-83
Number of pages11
JournalFEMS Immunology and Medical Microbiology
Volume32
Issue number1
DOIs
StatePublished - Dec 1 2001

Fingerprint

Salmonella typhimurium
Alcohol Drinking
Interferons
Macrophages
Infection
Nitric Oxide
Aptitude
Lipopolysaccharides
Interleukin-6
Tumor Necrosis Factor-alpha
Interferon Receptors
Adaptive Immunity
Innate Immunity
Endotoxins
Immunosuppression
Immune System
Ethanol
Animal Models
Cytokines
Incidence

Keywords

  • Ethanol
  • Interferon-γ
  • Interleukin-6
  • Lipopolysaccharide
  • Macrophage
  • Nitric oxide
  • Salmonella typhimurium
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Alcohol consumption is associated with alterations in macrophage responses to interferon-γ and infection by Salmonella typhimurium. / Sibley, Don A.; Osna, Natalia A; Kusynski, Charles; Wilkie, Linda; Jerrells, Thomas R.

In: FEMS Immunology and Medical Microbiology, Vol. 32, No. 1, 01.12.2001, p. 73-83.

Research output: Contribution to journalArticle

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abstract = "Abuse of ethanol (EtOH) by human beings and administration of EtOH to experimental animals has been shown to be associated with a suppression of the immune system. Consumption of EtOH has also been associated with an increased incidence and severity of infections of human beings and experimental animals, which has been attributed to the immunosuppression associated with EtOH consumption. It has been shown that EtOH also affects the function of macrophages (M{\O}), which are important effector cells in the innate and adaptive immune responses to infectious agents. The present studies were designed to investigate the effects of EtOH on M{\O} function with an animal model of EtOH consumption. The experiments reported in this paper were done with inflammatory M{\O} and were designed to determine the effects of EtOH on the ability of inflammatory M{\O} to respond to interferon-γ (IFN-γ) to control the intracellular growth of Salmonella typhimurium, as well as the production of proinflammatory cytokines and nitric oxide. The ability of M{\O} from EtOH-fed mice to respond to bacterial endotoxin (lipopolysaccharide (LPS)) and IFN-γ was also evaluated. M{\O} isolated from EtOH-fed mice did not respond as well to IFN-γ as M{\O} isolated from control mice as measured by control of S. typhimurium, as well as tumor necrosis factor (TNF) and nitric oxide production. Interleukin (IL)-6 production was not affected. Activation of M{\O} from EtOH-fed mice with LPS and IFN-γ produced levels of nitric oxide and TNF only slightly less than the levels seen in M{\O} from control mice, but a significant decrease in IL-6 was seen when M{\O} from EtOH-fed mice were stimulated with this combination. Flow cytometric analyses showed that IFN-γ receptor expression was not affected by EtOH. Together the data presented in this paper show that consumption of EtOH is associated with changes in inflammatory M{\O} responses to IFN-γ.",
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AU - Jerrells, Thomas R.

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