Albumin Enhanced Morphometric Image Analysis CLL

Matthew A Lunning, Vincent E. Zenger, Ricardo Dreyfuss, Maryalice Stetler-Stevenson, Margaret E. Rick, Therese A. White, Wyndham H. Wilson, Gerald E. Marti

Research output: Contribution to journalReview article

12 Citations (Scopus)

Abstract

Background: The heterogeneity of lymphocytes from patients with chronic lymphocytic leukemia (CLL) and blood film artifacts make morphologic subclassification of this disease difficult. Methods: We reviewed paired blood films prepared from ethylene-diamine-tetraacetic acid (ETDA) samples with and without bovine serum albumin (BSA) from 82 CLL patients. Group 1 adhered to NCCLS specifications for the preparations of EDTA blood films. Group 2 consisted of blood films containing EDTA and a 1:12 dilution of 22% BSA. Eight patients were selected for digital photomicroscopy and statistical analysis. Approximately 100 lymphocytes from each slide were digitally captured. Results: The mean cell area ± standard error was 127.8 μm2 ± 1.42 for (n = 793) for group 1 versus 100.7 μm2 ± 1.39 (n = 831) for group 2. The nuclear area was 88.9 μm2 ± 0.85 for group 1 versus 76.4 μm2 ± 0.83 for group 2. For the nuclear transmittance, the values were 97.6 ± 0.85 for group 1 and 104.1 ± 0.83 for group 2. The nuclear:cytoplasmic ratios were 0.71 ± 0.003 for group 1 and 0.78 ± 0.003 for group 2. All differences were statistically significant (P < 0.001). Conclusions: BSA addition results in the reduction of atypical lymphocytes and a decrease in smudge cells. BSA also decreases the lymphocyte area and nuclear area, whereas nuclear transmittance and nuclear:cytoplasmic ratio are increased. A standardized method of slide preparation would allow accurate interlaboratory comparison. The use of BSA may permit better implementation of the blood film-based subclassification of CLL and lead to a better correlation of morphology with cytogenetics and immunophenotyping.

Original languageEnglish (US)
Pages (from-to)7-14
Number of pages8
JournalCytometry Part B - Clinical Cytometry
Volume57
Issue number1
StatePublished - Jan 1 2004

Fingerprint

B-Cell Chronic Lymphocytic Leukemia
Bovine Serum Albumin
Albumins
Lymphocytes
Edetic Acid
Immunophenotyping
Diamines
Cytogenetics
Artifacts
Acids

Keywords

  • Atypical chronic lymphocytic leukemia
  • Atypical morphology
  • Chronic lymphocytic leukemia
  • Morphology
  • Morphometric analysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Lunning, M. A., Zenger, V. E., Dreyfuss, R., Stetler-Stevenson, M., Rick, M. E., White, T. A., ... Marti, G. E. (2004). Albumin Enhanced Morphometric Image Analysis CLL. Cytometry Part B - Clinical Cytometry, 57(1), 7-14.

Albumin Enhanced Morphometric Image Analysis CLL. / Lunning, Matthew A; Zenger, Vincent E.; Dreyfuss, Ricardo; Stetler-Stevenson, Maryalice; Rick, Margaret E.; White, Therese A.; Wilson, Wyndham H.; Marti, Gerald E.

In: Cytometry Part B - Clinical Cytometry, Vol. 57, No. 1, 01.01.2004, p. 7-14.

Research output: Contribution to journalReview article

Lunning, MA, Zenger, VE, Dreyfuss, R, Stetler-Stevenson, M, Rick, ME, White, TA, Wilson, WH & Marti, GE 2004, 'Albumin Enhanced Morphometric Image Analysis CLL', Cytometry Part B - Clinical Cytometry, vol. 57, no. 1, pp. 7-14.
Lunning MA, Zenger VE, Dreyfuss R, Stetler-Stevenson M, Rick ME, White TA et al. Albumin Enhanced Morphometric Image Analysis CLL. Cytometry Part B - Clinical Cytometry. 2004 Jan 1;57(1):7-14.
Lunning, Matthew A ; Zenger, Vincent E. ; Dreyfuss, Ricardo ; Stetler-Stevenson, Maryalice ; Rick, Margaret E. ; White, Therese A. ; Wilson, Wyndham H. ; Marti, Gerald E. / Albumin Enhanced Morphometric Image Analysis CLL. In: Cytometry Part B - Clinical Cytometry. 2004 ; Vol. 57, No. 1. pp. 7-14.
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abstract = "Background: The heterogeneity of lymphocytes from patients with chronic lymphocytic leukemia (CLL) and blood film artifacts make morphologic subclassification of this disease difficult. Methods: We reviewed paired blood films prepared from ethylene-diamine-tetraacetic acid (ETDA) samples with and without bovine serum albumin (BSA) from 82 CLL patients. Group 1 adhered to NCCLS specifications for the preparations of EDTA blood films. Group 2 consisted of blood films containing EDTA and a 1:12 dilution of 22{\%} BSA. Eight patients were selected for digital photomicroscopy and statistical analysis. Approximately 100 lymphocytes from each slide were digitally captured. Results: The mean cell area ± standard error was 127.8 μm2 ± 1.42 for (n = 793) for group 1 versus 100.7 μm2 ± 1.39 (n = 831) for group 2. The nuclear area was 88.9 μm2 ± 0.85 for group 1 versus 76.4 μm2 ± 0.83 for group 2. For the nuclear transmittance, the values were 97.6 ± 0.85 for group 1 and 104.1 ± 0.83 for group 2. The nuclear:cytoplasmic ratios were 0.71 ± 0.003 for group 1 and 0.78 ± 0.003 for group 2. All differences were statistically significant (P < 0.001). Conclusions: BSA addition results in the reduction of atypical lymphocytes and a decrease in smudge cells. BSA also decreases the lymphocyte area and nuclear area, whereas nuclear transmittance and nuclear:cytoplasmic ratio are increased. A standardized method of slide preparation would allow accurate interlaboratory comparison. The use of BSA may permit better implementation of the blood film-based subclassification of CLL and lead to a better correlation of morphology with cytogenetics and immunophenotyping.",
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AU - Rick, Margaret E.

AU - White, Therese A.

AU - Wilson, Wyndham H.

AU - Marti, Gerald E.

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N2 - Background: The heterogeneity of lymphocytes from patients with chronic lymphocytic leukemia (CLL) and blood film artifacts make morphologic subclassification of this disease difficult. Methods: We reviewed paired blood films prepared from ethylene-diamine-tetraacetic acid (ETDA) samples with and without bovine serum albumin (BSA) from 82 CLL patients. Group 1 adhered to NCCLS specifications for the preparations of EDTA blood films. Group 2 consisted of blood films containing EDTA and a 1:12 dilution of 22% BSA. Eight patients were selected for digital photomicroscopy and statistical analysis. Approximately 100 lymphocytes from each slide were digitally captured. Results: The mean cell area ± standard error was 127.8 μm2 ± 1.42 for (n = 793) for group 1 versus 100.7 μm2 ± 1.39 (n = 831) for group 2. The nuclear area was 88.9 μm2 ± 0.85 for group 1 versus 76.4 μm2 ± 0.83 for group 2. For the nuclear transmittance, the values were 97.6 ± 0.85 for group 1 and 104.1 ± 0.83 for group 2. The nuclear:cytoplasmic ratios were 0.71 ± 0.003 for group 1 and 0.78 ± 0.003 for group 2. All differences were statistically significant (P < 0.001). Conclusions: BSA addition results in the reduction of atypical lymphocytes and a decrease in smudge cells. BSA also decreases the lymphocyte area and nuclear area, whereas nuclear transmittance and nuclear:cytoplasmic ratio are increased. A standardized method of slide preparation would allow accurate interlaboratory comparison. The use of BSA may permit better implementation of the blood film-based subclassification of CLL and lead to a better correlation of morphology with cytogenetics and immunophenotyping.

AB - Background: The heterogeneity of lymphocytes from patients with chronic lymphocytic leukemia (CLL) and blood film artifacts make morphologic subclassification of this disease difficult. Methods: We reviewed paired blood films prepared from ethylene-diamine-tetraacetic acid (ETDA) samples with and without bovine serum albumin (BSA) from 82 CLL patients. Group 1 adhered to NCCLS specifications for the preparations of EDTA blood films. Group 2 consisted of blood films containing EDTA and a 1:12 dilution of 22% BSA. Eight patients were selected for digital photomicroscopy and statistical analysis. Approximately 100 lymphocytes from each slide were digitally captured. Results: The mean cell area ± standard error was 127.8 μm2 ± 1.42 for (n = 793) for group 1 versus 100.7 μm2 ± 1.39 (n = 831) for group 2. The nuclear area was 88.9 μm2 ± 0.85 for group 1 versus 76.4 μm2 ± 0.83 for group 2. For the nuclear transmittance, the values were 97.6 ± 0.85 for group 1 and 104.1 ± 0.83 for group 2. The nuclear:cytoplasmic ratios were 0.71 ± 0.003 for group 1 and 0.78 ± 0.003 for group 2. All differences were statistically significant (P < 0.001). Conclusions: BSA addition results in the reduction of atypical lymphocytes and a decrease in smudge cells. BSA also decreases the lymphocyte area and nuclear area, whereas nuclear transmittance and nuclear:cytoplasmic ratio are increased. A standardized method of slide preparation would allow accurate interlaboratory comparison. The use of BSA may permit better implementation of the blood film-based subclassification of CLL and lead to a better correlation of morphology with cytogenetics and immunophenotyping.

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