Akt inhibitor MK-2206 promotes anti-tumor activity and cell death by modulation of AIF and Ezrin in colorectal cancer

Ekta Agarwal, Anathbandhu Chaudhuri, Premila D. Leiphrakpam, Katie L. Haferbier, Michael G. Brattain, Sanjib Chowdhury

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: There is extensive evidence for the role of aberrant cell survival signaling mechanisms in cancer progression and metastasis. Akt is a major component of cell survival-signaling mechanisms in several types of cancer. It has been shown that activated Akt stabilizes XIAP by S87 phosphorylation leading to survivin/XIAP complex formation, caspase inhibition and cytoprotection of cancer cells. We have reported that TGFβ/PKA/PP2A-mediated tumor suppressor signaling regulates Akt phosphorylation in association with the dissociation of survivin/XIAP complexes leading to inhibition of stress-dependent induction of cell survival.Methods: IGF1R-dependent colon cancer cells (GEO and CBS) were used for the study. Effects on cell proliferation and cell death were determined in the presence of MK-2206. Xenograft studies were performed to determine the effect of MK-2206 on tumor volume. The effect on various cell death markers such as XIAP, survivin, AIF, Ezrin, pEzrin was determined by western blot analysis. Graph pad 5.0 was used for statistical analysis. P < 0.05 was considered significant.Results: We characterized the mechanisms by which a novel Akt kinase inhibitor MK-2206 induced cell death in IGF1R-dependent colorectal cancer (CRC) cells with upregulated PI3K/Akt signaling in response to IGF1R activation. MK-2206 treatment generated a significant reduction in tumor growth in vivo and promoted cell death through two mechanisms. This is the first report demonstrating that Akt inactivation by MK-2206 leads to induction of and mitochondria-to-nuclear localization of the Apoptosis Inducing Factor (AIF), which is involved in caspase-independent cell death. We also observed that exposure to MK-2206 dephosphorylated Ezrin at the T567 site leading to the disruption of Akt-pEzrin-XIAP cell survival signaling. Ezrin phosphorylation at this site has been associated with malignant progression in solid tumors.Conclusion: The identification of these 2 novel mechanisms leading to induction of cell death indicates MK-2206 might be a potential clinical candidate for therapeutic targeting of the subset of IGF1R-dependent cancers in CRC.

Original languageEnglish (US)
Article number145
JournalBMC cancer
Volume14
Issue number1
DOIs
StatePublished - Mar 1 2014

Fingerprint

Apoptosis Inducing Factor
Colorectal Neoplasms
Cell Death
Cell Survival
Neoplasms
Phosphorylation
Caspases
Cytoprotection
MK 2206
ezrin
Tumor Burden
Phosphatidylinositol 3-Kinases
Heterografts
Colonic Neoplasms
Mitochondria
Phosphotransferases
Western Blotting
Cell Proliferation
Neoplasm Metastasis
Growth

Keywords

  • AIF
  • Akt inhibitor MK-2206
  • Akt isoforms
  • Cell death
  • Cell survival
  • Ezrin T567
  • PI3K
  • Survivin
  • XIAP

ASJC Scopus subject areas

  • Genetics
  • Oncology
  • Cancer Research

Cite this

Agarwal, E., Chaudhuri, A., Leiphrakpam, P. D., Haferbier, K. L., Brattain, M. G., & Chowdhury, S. (2014). Akt inhibitor MK-2206 promotes anti-tumor activity and cell death by modulation of AIF and Ezrin in colorectal cancer. BMC cancer, 14(1), [145]. https://doi.org/10.1186/1471-2407-14-145

Akt inhibitor MK-2206 promotes anti-tumor activity and cell death by modulation of AIF and Ezrin in colorectal cancer. / Agarwal, Ekta; Chaudhuri, Anathbandhu; Leiphrakpam, Premila D.; Haferbier, Katie L.; Brattain, Michael G.; Chowdhury, Sanjib.

In: BMC cancer, Vol. 14, No. 1, 145, 01.03.2014.

Research output: Contribution to journalArticle

Agarwal, E, Chaudhuri, A, Leiphrakpam, PD, Haferbier, KL, Brattain, MG & Chowdhury, S 2014, 'Akt inhibitor MK-2206 promotes anti-tumor activity and cell death by modulation of AIF and Ezrin in colorectal cancer', BMC cancer, vol. 14, no. 1, 145. https://doi.org/10.1186/1471-2407-14-145
Agarwal, Ekta ; Chaudhuri, Anathbandhu ; Leiphrakpam, Premila D. ; Haferbier, Katie L. ; Brattain, Michael G. ; Chowdhury, Sanjib. / Akt inhibitor MK-2206 promotes anti-tumor activity and cell death by modulation of AIF and Ezrin in colorectal cancer. In: BMC cancer. 2014 ; Vol. 14, No. 1.
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AU - Leiphrakpam, Premila D.

AU - Haferbier, Katie L.

AU - Brattain, Michael G.

AU - Chowdhury, Sanjib

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N2 - Background: There is extensive evidence for the role of aberrant cell survival signaling mechanisms in cancer progression and metastasis. Akt is a major component of cell survival-signaling mechanisms in several types of cancer. It has been shown that activated Akt stabilizes XIAP by S87 phosphorylation leading to survivin/XIAP complex formation, caspase inhibition and cytoprotection of cancer cells. We have reported that TGFβ/PKA/PP2A-mediated tumor suppressor signaling regulates Akt phosphorylation in association with the dissociation of survivin/XIAP complexes leading to inhibition of stress-dependent induction of cell survival.Methods: IGF1R-dependent colon cancer cells (GEO and CBS) were used for the study. Effects on cell proliferation and cell death were determined in the presence of MK-2206. Xenograft studies were performed to determine the effect of MK-2206 on tumor volume. The effect on various cell death markers such as XIAP, survivin, AIF, Ezrin, pEzrin was determined by western blot analysis. Graph pad 5.0 was used for statistical analysis. P < 0.05 was considered significant.Results: We characterized the mechanisms by which a novel Akt kinase inhibitor MK-2206 induced cell death in IGF1R-dependent colorectal cancer (CRC) cells with upregulated PI3K/Akt signaling in response to IGF1R activation. MK-2206 treatment generated a significant reduction in tumor growth in vivo and promoted cell death through two mechanisms. This is the first report demonstrating that Akt inactivation by MK-2206 leads to induction of and mitochondria-to-nuclear localization of the Apoptosis Inducing Factor (AIF), which is involved in caspase-independent cell death. We also observed that exposure to MK-2206 dephosphorylated Ezrin at the T567 site leading to the disruption of Akt-pEzrin-XIAP cell survival signaling. Ezrin phosphorylation at this site has been associated with malignant progression in solid tumors.Conclusion: The identification of these 2 novel mechanisms leading to induction of cell death indicates MK-2206 might be a potential clinical candidate for therapeutic targeting of the subset of IGF1R-dependent cancers in CRC.

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