Agricultural waste materials enhance protease production by Bacillus subtilis B22 in submerged fermentation under blue light-emitting diodes

Punniyakotti Elumalai, Jeong Muk Lim, Yool Jin Park, Min Cho, Patrick J. Shea, Byung Taek Oh

Research output: Contribution to journalArticle

Abstract

Bacillus bacteria have major utility in large-scale production of industrial enzymes, among which proteases have particular importance. B. subtilis B22, an aerobic and chemotrophic strain, was isolated from kimchi and identified by 16S rRNA gene sequencing. Extracellular protease production was determined in basic medium, with 1% (w/v) casein as substrate, by submerged fermentation at 37 °C under blue, green, red and white light-emitting diodes (LEDs), white fluorescent light and darkness. Fermentation under blue LEDs maximized protease production (110.79 ± 1.8 U/mL at 24 h). Various agricultural waste products enhanced production and groundnut oil cake yielded the most protease (334 ± 1.8 U/mL at 72 h). Activity and stability of the purified protease were optimum at pH 7–10 and 20–60 °C. Activity increased in the presence of Ca2+, Mg2+ and Mn2+, while Fe2+, Zn2+, Co2+ and Cu2+ moderated activity, and Ni2+ and Hg2+ inhibited activity. Activity was high (98%) in the presence of ethylenediaminetetraacetic acid (EDTA) but inhibited by phenylmethanesulfonyl fluoride (PMSF). The protease was unaffected by nonionic surfactants, tolerated an anionic surfactant and oxidizing agents, and was compatible with multiple organic solvents. These properties suggest utility of protease produced by B. subtilis B22 under blue LEDs for industrial applications.

Original languageEnglish (US)
JournalBioprocess and Biosystems Engineering
DOIs
StateAccepted/In press - Jan 1 2020

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Agricultural wastes
Bacilli
Bacillus subtilis
Fermentation
Light emitting diodes
Peptide Hydrolases
Light
Surface-Active Agents
Phenylmethylsulfonyl Fluoride
Waste Products
Casein
Anionic surfactants
Darkness
Ethylenediaminetetraacetic acid
Nonionic surfactants
Caseins
rRNA Genes
Oxidants
Edetic Acid
Organic solvents

Keywords

  • Agricultural waste products
  • B. subtilis
  • Blue LEDs
  • Protease
  • Submerged fermentation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Agricultural waste materials enhance protease production by Bacillus subtilis B22 in submerged fermentation under blue light-emitting diodes. / Elumalai, Punniyakotti; Lim, Jeong Muk; Park, Yool Jin; Cho, Min; Shea, Patrick J.; Oh, Byung Taek.

In: Bioprocess and Biosystems Engineering, 01.01.2020.

Research output: Contribution to journalArticle

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