Aging synaptic mitochondria exhibit dynamic proteomic changes while maintaining bioenergetic function

Kelly L. Stauch, Phillip R. Purnell, Howard S Fox

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Aging correlates with a progressive impairment of mitochondrial homeostasis and is an influential factor for several forms of neurodegeneration. However, the mechanisms underlying age-related alterations in synaptosomal mitochondria, a neuronal mitochondria population highly susceptible to insults and critical for brain function, remain incompletely understood. Therefore this study investigates the synaptic mitochondrial proteomic and bioenergetic alterations that occur with age. The utilization of a state of the art quantitative proteomics approach allowed for the comparison of protein expression levels in synaptic mitochondria isolated from 5 (mature), 12 (old), and 24 (aged) month old mice. During the process of aging we find that dynamic proteomic alterations occur in synaptic mitochondria. Despite direct (mitochondrial DNA deletions) and indirect (increased antioxidant protein levels) signs of mitochondrial damage in the aged mice, there was an overall maintenance of mitochondrial function. Therefore the synaptic mitochondrial proteomic changes that occur with aging correlate with preservation of synaptic mitochondrial function.

Original languageEnglish (US)
Pages (from-to)320-334
Number of pages15
JournalAging
Volume6
Issue number4
DOIs
StatePublished - 2014

    Fingerprint

Keywords

  • Aging
  • Bioenergetics
  • Hormesis
  • Proteomics
  • Synaptic mitochondria

ASJC Scopus subject areas

  • Aging
  • Cell Biology

Cite this