Age-related changes in the gut microbiota of the Chinese giant salamander (Andrias davidianus)

Mengjie Zhang, Sarah Gaughan, Qing Chang, Hua Chen, Guoqing Lu, Xungang Wang, Liangliang Xu, Lifeng Zhu, Jianping Jiang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The composition of the intestinal microbial community may vary across developmental stages. In this study, we explored how this microbial community shifted along the intestinal tract of the Chinese giant salamander (Andrias davidianus) at various ages. Next-generation sequencing was used to sequence the bacterial 16S rRNA gene from different kind of samples, including the stomach, duodenum, ileum, and rectum. The highest mean relative abundance of the bacterial community in the gastrointestinal tract shifted in relation to age: within the first year, Bacteroidetes (47.76%) dominated the gut microbiome, whereas Proteobacteria was the most dominant at age 2 (32.88%) and age 3 (30.78%), and finally, Firmicutes was the most dominant at age 4 (34.70%). The overall richness of the gut bacterial community also generally increased from age 2 to 4. Hierarchical cluster analysis revealed that the gut microbiome at age 2 had greater variability than that at either age 3 or 4, likely representing a shift in diet from yolk or redworms as a juvenile to shrimp or crab as an adult. As these salamanders develop, their gastrointestinal tracts increase in complexity, and this compartmentalization may also facilitate an increase in microbial gut diversity.

Original languageEnglish (US)
Article numbere00778
JournalMicrobiologyOpen
Volume8
Issue number7
DOIs
StatePublished - Jul 2019

Fingerprint

Urodela
Gastrointestinal Tract
Bacteroidetes
Proteobacteria
Ileum
rRNA Genes
Duodenum
Rectum
Cluster Analysis
Stomach
Diet
Gastrointestinal Microbiome
Firmicutes

Keywords

  • Chinese giant salamander
  • age
  • gastrointestinal tract
  • microbial community changes
  • nutritional source shift

ASJC Scopus subject areas

  • Microbiology

Cite this

Age-related changes in the gut microbiota of the Chinese giant salamander (Andrias davidianus). / Zhang, Mengjie; Gaughan, Sarah; Chang, Qing; Chen, Hua; Lu, Guoqing; Wang, Xungang; Xu, Liangliang; Zhu, Lifeng; Jiang, Jianping.

In: MicrobiologyOpen, Vol. 8, No. 7, e00778, 07.2019.

Research output: Contribution to journalArticle

Zhang, M, Gaughan, S, Chang, Q, Chen, H, Lu, G, Wang, X, Xu, L, Zhu, L & Jiang, J 2019, 'Age-related changes in the gut microbiota of the Chinese giant salamander (Andrias davidianus)', MicrobiologyOpen, vol. 8, no. 7, e00778. https://doi.org/10.1002/mbo3.778
Zhang, Mengjie ; Gaughan, Sarah ; Chang, Qing ; Chen, Hua ; Lu, Guoqing ; Wang, Xungang ; Xu, Liangliang ; Zhu, Lifeng ; Jiang, Jianping. / Age-related changes in the gut microbiota of the Chinese giant salamander (Andrias davidianus). In: MicrobiologyOpen. 2019 ; Vol. 8, No. 7.
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