AFM visualization of protein-DNA interactions

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Proteins are the principal mediators of DNA processes and organization. Enzymes and regulatory proteins perform cellular processes, such as replication, transcription, and recombination; while architectural proteins are involved in the organization of DNA within the cell into structures, such as chromatin. DNA is a long polymeric molecule, ranging in size of several thousand monomeric units (bacteriophage genome) to a million base pairs (eukaryotic genome); therefore, nanoscale imaging tools are instrumental in understanding the mechanisms of genetic processes and intracellular DNA organization. Historically, electron microscopy (EM) was widely employed to study the various properties of DNA. Of particular note, EM imaging was critical in proving the nucleosomal organization of DNA within the cell by graphically illustrating the beads-on-a-string model of unfolded chromatin and elucidating the higher order structure of chromatin [see Watson (2008) and numerous references therein]. The advent of atomic force microscopy (AFM), and the application of this nanoimaging technique to molecular biology, opens new prospects for studies of protein-DNA complexes. AFM technology allows for a gentle sample preparation, easing concerns about sample preservation. Moreover, AFM is capable of imaging samples in fully hydrated states by scanning in aqueous solutions. Therefore, in addition to nanoscale static structural data of protein-DNA complexes, AFM visualizes the dynamics of conformational transitions of DNA and various nucleoprotein complexes.

Original languageEnglish (US)
Title of host publicationSingle-molecule Studies of Proteins
PublisherSpringer New York
Pages97-117
Number of pages21
ISBN (Electronic)9781461449218
ISBN (Print)9781461449201
DOIs
StatePublished - Jan 1 2013

Fingerprint

Atomic Force Microscopy
Atomic force microscopy
Visualization
DNA
Proteins
Chromatin
Imaging techniques
Electron microscopy
Electron Microscopy
Genes
Genetic Phenomena
Genome
Molecular biology
Bacteriophages
Nucleoproteins
Transcription
Base Pairing
Genetic Recombination
Molecular Biology
Technology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lyubchenko, Y. L. (2013). AFM visualization of protein-DNA interactions. In Single-molecule Studies of Proteins (pp. 97-117). Springer New York. https://doi.org/10.1007/978-1-4614-4921-8_4

AFM visualization of protein-DNA interactions. / Lyubchenko, Yuri L.

Single-molecule Studies of Proteins. Springer New York, 2013. p. 97-117.

Research output: Chapter in Book/Report/Conference proceedingChapter

Lyubchenko, YL 2013, AFM visualization of protein-DNA interactions. in Single-molecule Studies of Proteins. Springer New York, pp. 97-117. https://doi.org/10.1007/978-1-4614-4921-8_4
Lyubchenko YL. AFM visualization of protein-DNA interactions. In Single-molecule Studies of Proteins. Springer New York. 2013. p. 97-117 https://doi.org/10.1007/978-1-4614-4921-8_4
Lyubchenko, Yuri L. / AFM visualization of protein-DNA interactions. Single-molecule Studies of Proteins. Springer New York, 2013. pp. 97-117
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