AFM Identification of Beetle Exocuticle: Bouligand Structure and Nanofiber Anisotropic Elastic Properties

Ruiguo Yang, Alireza Zaheri, Wei Gao, Cheryl Hayashi, Horacio D. Espinosa

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

One of the common architectures in natural materials is the helicoidal (Bouligand) structure, where fiber layers twist around a helical screw. Despite the many studies that have shown the existence of Bouligand structures, methods for nanoscale structural characterization and identification of fiber mechanical properties remain to be developed. In this study, we used the exocuticle of Cotinis mutabilis (a beetle in the Cetoniinae) as a model material to develop a new experimental-theoretical methodology that combines atomic force microscopy-based nanoindentation and anisotropic contact mechanics analysis. Using such methodology, we studied both the helicoidal structure and the mechanical properties of its constituent fibers. The twist angle between the layers was found to be in the range of 12°–18° with a pitch size of 220 nm for the helicoidal pattern. In addition, the constituent fiber diameter was measured to be approximately 20 nm, which is consistent with the fiber diameters found in helicoids of other arthropod species. The longitudinal, transverse, and shear modulus of the nanofiber were determined to be 710 MPa, 70 MPa, and 90 MPa, respectively. The established experimental-theoretical methodology promises to be a useful tool for nanoscale characterization of helicoidal and other structures found in biological materials.

Original languageEnglish (US)
Article number1603993
JournalAdvanced Functional Materials
Volume27
Issue number6
DOIs
StatePublished - Feb 10 2017

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Keywords

  • atomic force microscopy
  • beetles
  • biomimicking
  • mechanical properties
  • natural materials

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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