Affinity chromatography of α-amylase from Bacillus licheniformis

Damodara Rao Mendu, B. V.V. Ratnam, A. Purnima, C. Ayyanna

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

An affinity chromatographic method with a novel eluant from Bacillus licheniformis is described. α-amylase was bound to starch, starch-celite, starch-Sepharose columns and the bound α-amylase was rapidly eluted with 2% (w/v) white dextrin. The binding capacity of α-amylase to starch column is 380 μmol/g of starch. The purified enzyme showed a single polypeptide on SDS-polyacrylamide gel electrophoresis with a molecular weight of 58 kD. The specificity of purified enzyme was confirmed by immunodiffusion, immunoelectrophoresis. Single radial immunodiffusion and western blotting studies analyzed the synthesis of enzyme at different time points.

Original languageEnglish (US)
Pages (from-to)712-717
Number of pages6
JournalEnzyme and Microbial Technology
Volume37
Issue number7
DOIs
StatePublished - Dec 1 2005

Fingerprint

Affinity chromatography
Amylases
Bacilli
Starch
Affinity Chromatography
Enzymes
Immunodiffusion
Diatomaceous Earth
Immunoelectrophoresis
Polypeptides
Polyacrylates
Electrophoresis
Sepharose
Polyacrylamide Gel Electrophoresis
Gels
Molecular Weight
Western Blotting
Molecular weight
Bacillus licheniformis
Peptides

Keywords

  • Affinity chromatography
  • Bacillus licheniformis
  • Immunological characterization
  • α-Amylase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Affinity chromatography of α-amylase from Bacillus licheniformis. / Mendu, Damodara Rao; Ratnam, B. V.V.; Purnima, A.; Ayyanna, C.

In: Enzyme and Microbial Technology, Vol. 37, No. 7, 01.12.2005, p. 712-717.

Research output: Contribution to journalArticle

Mendu, Damodara Rao ; Ratnam, B. V.V. ; Purnima, A. ; Ayyanna, C. / Affinity chromatography of α-amylase from Bacillus licheniformis. In: Enzyme and Microbial Technology. 2005 ; Vol. 37, No. 7. pp. 712-717.
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