Aerobic biodegradation of OCDD by P. Mendocina NSYSU

Effectiveness and gene inducement studies

J. L. Lin, W. C. Lin, J. K. Liu, R. Y. Surampalli, Tian C Zhang, C. M. Kao

Research output: Contribution to journalArticle

Abstract

The goals of this study were to assess the effectiveness of (1) enhancing octachlorinated dibenzo-p-dioxin (OCDD) biodegradation under aerobic conditions by Pseudomonas mendocina NSYSU (P. mendocina NSYSU) with the addition of lecithin, and (2) inducing OCDD ring-cleavage genes by pentachlorophenol (PCP) and OCDD addition. P. mendocina NSYSU could biodegrade OCDD via aerobic cometabolism and lecithin was used as a primary substrate. Approximately 74 and 67% of OCDD biodegradation was observed after 60 days of incubation with lecithin and glucose supplement, respectively. Lecithin was also used as the solubilization additive resulting in OCDD solubilization and enhanced bioavailability of OCDD to P. mendocina NSYSU. Two intradiol and extradiol ringcleavage dioxygenase genes (Pmen-0474 and Pmen-2526) were identified from gene analyses. Gene concentration was significantly enhanced after the inducement by PCP and OCDD. Higher gene inducement efficiency was obtained using PCP as the inducer, and Pmen-2526 played a more important role in OCDD biodegradation.

Original languageEnglish (US)
Pages (from-to)2113-2121
Number of pages9
JournalWater Environment Research
Volume89
Issue number12
DOIs
StatePublished - Dec 1 2017

Fingerprint

Lecithin
Biodegradation
dioxin
biodegradation
Genes
gene
Lecithins
Pentachlorophenol
PCP
solubilization
Glucose
dibenzo(1,4)dioxin
oxic conditions
Substrates
cleavage
bioavailability
glucose
incubation
substrate

Keywords

  • Aerobic cometabolic biodegradation
  • Gene inducement
  • Octachlorinated dibenzo-p-dioxin
  • Pseudomonas mendocin
  • Ring-cleavage dioxygenase

ASJC Scopus subject areas

  • Environmental Chemistry
  • Ecological Modeling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Cite this

Aerobic biodegradation of OCDD by P. Mendocina NSYSU : Effectiveness and gene inducement studies. / Lin, J. L.; Lin, W. C.; Liu, J. K.; Surampalli, R. Y.; Zhang, Tian C; Kao, C. M.

In: Water Environment Research, Vol. 89, No. 12, 01.12.2017, p. 2113-2121.

Research output: Contribution to journalArticle

Lin, J. L. ; Lin, W. C. ; Liu, J. K. ; Surampalli, R. Y. ; Zhang, Tian C ; Kao, C. M. / Aerobic biodegradation of OCDD by P. Mendocina NSYSU : Effectiveness and gene inducement studies. In: Water Environment Research. 2017 ; Vol. 89, No. 12. pp. 2113-2121.
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