Advanced analysis of biotin metabolites in body fluids allows a more accurate measurement of biotin bioavailability and metabolism in humans

Janos Zempleni, Donald M. Mock

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In previous studies, the bioavailability of biotin in humans was estimated from the recovery of biotin in urine; urinary biotin was measured by microbial growth assays or assays of avidin-binding activity. These assays underestimate concentrations of biotin metabolites, which originate from β- oxidation, sulfur oxidation or a combination: We have developed an HPLC/avidin-binding assay that is specific for biotin and its metabolites. With the use of the HPLC/avidin-binding assay, TLC and derivatization with p- dimethylaminocinnamaldehyde we have identified and quantitated biotin and metabolites in urine from six healthy adults. Of that total, biotin accounted for 32 ± 12%, bisnorbiotin for 52 ± 15%, bisnorbiotin methyl ketone for 7.9 ± 5.8%, biotin-d,l-sulfoxide for 4.0 ± 3.2% and biotin sulfone for 3.6 ± 1.9%. After intravenous administration of 18.4 μmol of biotin, the urinary excretion of biotin metabolites increased 21-130 times above baseline values. Because the biliary excretion of biotin is quantitatively minor (1.9 ± 0.2% of an intravenous [14C]biotin dose in rats), intravenously administered biotin is not exposed to intestinal microorganisms. Thus we conclude that biotin metabolites in human urine originate from biotin catabolism in human tissues rather than biotin catabolism by intestinal microorganisms. With the use of the HPLC/avidin-binding assay, we estimated the bioavailability of biotin in adults from the urinary excretion of biotin and metabolites after ingestion of 2.1, 8.2 and 81.9 μmol of biotin. These data provide evidence that biotin is nearly completely absorbed.

Original languageEnglish (US)
Pages (from-to)494S-497S
JournalJournal of Nutrition
Volume129
Issue number2 SUPPL.
StatePublished - Feb 15 1999

Fingerprint

body fluids
Body Fluids
biotin
Biotin
Biological Availability
bioavailability
metabolites
metabolism
Avidin
avidin
assays
sulfoxide
urine
High Pressure Liquid Chromatography
excretion
Urine
intestinal microorganisms
oxidation

Keywords

  • Analysis
  • Bioavailability
  • Biotin
  • Biotin metabolites
  • Humans

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Advanced analysis of biotin metabolites in body fluids allows a more accurate measurement of biotin bioavailability and metabolism in humans. / Zempleni, Janos; Mock, Donald M.

In: Journal of Nutrition, Vol. 129, No. 2 SUPPL., 15.02.1999, p. 494S-497S.

Research output: Contribution to journalArticle

@article{11e5ae676ccc4bae9a457fa9f6464035,
title = "Advanced analysis of biotin metabolites in body fluids allows a more accurate measurement of biotin bioavailability and metabolism in humans",
abstract = "In previous studies, the bioavailability of biotin in humans was estimated from the recovery of biotin in urine; urinary biotin was measured by microbial growth assays or assays of avidin-binding activity. These assays underestimate concentrations of biotin metabolites, which originate from β- oxidation, sulfur oxidation or a combination: We have developed an HPLC/avidin-binding assay that is specific for biotin and its metabolites. With the use of the HPLC/avidin-binding assay, TLC and derivatization with p- dimethylaminocinnamaldehyde we have identified and quantitated biotin and metabolites in urine from six healthy adults. Of that total, biotin accounted for 32 ± 12{\%}, bisnorbiotin for 52 ± 15{\%}, bisnorbiotin methyl ketone for 7.9 ± 5.8{\%}, biotin-d,l-sulfoxide for 4.0 ± 3.2{\%} and biotin sulfone for 3.6 ± 1.9{\%}. After intravenous administration of 18.4 μmol of biotin, the urinary excretion of biotin metabolites increased 21-130 times above baseline values. Because the biliary excretion of biotin is quantitatively minor (1.9 ± 0.2{\%} of an intravenous [14C]biotin dose in rats), intravenously administered biotin is not exposed to intestinal microorganisms. Thus we conclude that biotin metabolites in human urine originate from biotin catabolism in human tissues rather than biotin catabolism by intestinal microorganisms. With the use of the HPLC/avidin-binding assay, we estimated the bioavailability of biotin in adults from the urinary excretion of biotin and metabolites after ingestion of 2.1, 8.2 and 81.9 μmol of biotin. These data provide evidence that biotin is nearly completely absorbed.",
keywords = "Analysis, Bioavailability, Biotin, Biotin metabolites, Humans",
author = "Janos Zempleni and Mock, {Donald M.}",
year = "1999",
month = "2",
day = "15",
language = "English (US)",
volume = "129",
pages = "494S--497S",
journal = "The Journal of nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "2 SUPPL.",

}

TY - JOUR

T1 - Advanced analysis of biotin metabolites in body fluids allows a more accurate measurement of biotin bioavailability and metabolism in humans

AU - Zempleni, Janos

AU - Mock, Donald M.

PY - 1999/2/15

Y1 - 1999/2/15

N2 - In previous studies, the bioavailability of biotin in humans was estimated from the recovery of biotin in urine; urinary biotin was measured by microbial growth assays or assays of avidin-binding activity. These assays underestimate concentrations of biotin metabolites, which originate from β- oxidation, sulfur oxidation or a combination: We have developed an HPLC/avidin-binding assay that is specific for biotin and its metabolites. With the use of the HPLC/avidin-binding assay, TLC and derivatization with p- dimethylaminocinnamaldehyde we have identified and quantitated biotin and metabolites in urine from six healthy adults. Of that total, biotin accounted for 32 ± 12%, bisnorbiotin for 52 ± 15%, bisnorbiotin methyl ketone for 7.9 ± 5.8%, biotin-d,l-sulfoxide for 4.0 ± 3.2% and biotin sulfone for 3.6 ± 1.9%. After intravenous administration of 18.4 μmol of biotin, the urinary excretion of biotin metabolites increased 21-130 times above baseline values. Because the biliary excretion of biotin is quantitatively minor (1.9 ± 0.2% of an intravenous [14C]biotin dose in rats), intravenously administered biotin is not exposed to intestinal microorganisms. Thus we conclude that biotin metabolites in human urine originate from biotin catabolism in human tissues rather than biotin catabolism by intestinal microorganisms. With the use of the HPLC/avidin-binding assay, we estimated the bioavailability of biotin in adults from the urinary excretion of biotin and metabolites after ingestion of 2.1, 8.2 and 81.9 μmol of biotin. These data provide evidence that biotin is nearly completely absorbed.

AB - In previous studies, the bioavailability of biotin in humans was estimated from the recovery of biotin in urine; urinary biotin was measured by microbial growth assays or assays of avidin-binding activity. These assays underestimate concentrations of biotin metabolites, which originate from β- oxidation, sulfur oxidation or a combination: We have developed an HPLC/avidin-binding assay that is specific for biotin and its metabolites. With the use of the HPLC/avidin-binding assay, TLC and derivatization with p- dimethylaminocinnamaldehyde we have identified and quantitated biotin and metabolites in urine from six healthy adults. Of that total, biotin accounted for 32 ± 12%, bisnorbiotin for 52 ± 15%, bisnorbiotin methyl ketone for 7.9 ± 5.8%, biotin-d,l-sulfoxide for 4.0 ± 3.2% and biotin sulfone for 3.6 ± 1.9%. After intravenous administration of 18.4 μmol of biotin, the urinary excretion of biotin metabolites increased 21-130 times above baseline values. Because the biliary excretion of biotin is quantitatively minor (1.9 ± 0.2% of an intravenous [14C]biotin dose in rats), intravenously administered biotin is not exposed to intestinal microorganisms. Thus we conclude that biotin metabolites in human urine originate from biotin catabolism in human tissues rather than biotin catabolism by intestinal microorganisms. With the use of the HPLC/avidin-binding assay, we estimated the bioavailability of biotin in adults from the urinary excretion of biotin and metabolites after ingestion of 2.1, 8.2 and 81.9 μmol of biotin. These data provide evidence that biotin is nearly completely absorbed.

KW - Analysis

KW - Bioavailability

KW - Biotin

KW - Biotin metabolites

KW - Humans

UR - http://www.scopus.com/inward/record.url?scp=0032958829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032958829&partnerID=8YFLogxK

M3 - Article

C2 - 10064316

AN - SCOPUS:0032958829

VL - 129

SP - 494S-497S

JO - The Journal of nutrition

JF - The Journal of nutrition

SN - 0022-3166

IS - 2 SUPPL.

ER -