Administration of gm-csf to breast cancer patients mobilizes low numbers of tumor cells into leukapheresis harvests

S. Tarantolo, M. Travstman, Elizabeth Cecile Reed, B. Murohv, S. Mann, A. Kessinqer, John G Sharp

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Brugger et al (1994) reported that the administration of chemotherapy followed by G-CSF to patients (pts) with breast and lung cancer caused epithelial cells to appear in the circulation at a time dependent on whether bone marrow was involved with tumor. In this circumstance, it is, unclear if vascular damage caused by the chemotherapy contributes to the release of tumor cells. Consequently, we have evaluated mobilization of breast cancer cells into apheresis harvests in 15 pts with metastatic breast cancer following administration of GM-CSF alone. The tumor cells were detected by both immunocytochemistry(ICC) and RT-PCR to cytokeratins 8, 18 and 19. Blood and bone marrow were sampled prior to apheresis harvests and during cytokine administration, as well as, the pooled frozen sample administered to the patient and a blood sample immediately post-transplant. We observed positive apheresis harvests in pts with negative blood and marrow prior to mobilization. These results demonstrate that breast cancer cells are mobilized in low numbers, into leukapheresis harvests following the administration of cytokine alone and some tumor cells are likely to be re-infused with the harvest. However, the clinical significance of reinfusion of small numbers of mobilized tumor cells remains to be determined.

Original languageEnglish (US)
Number of pages1
JournalExperimental Hematology
Volume24
Issue number9
StatePublished - Dec 1 1996

Fingerprint

Leukapheresis
Cell Count
Breast Neoplasms
Blood Component Removal
Neoplasms
Bone Marrow
Keratin-8
Cytokines
Keratin-18
Keratin-19
Drug Therapy
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Blood Vessels
Lung Neoplasms
Epithelial Cells
Immunohistochemistry
Transplants
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Administration of gm-csf to breast cancer patients mobilizes low numbers of tumor cells into leukapheresis harvests. / Tarantolo, S.; Travstman, M.; Reed, Elizabeth Cecile; Murohv, B.; Mann, S.; Kessinqer, A.; Sharp, John G.

In: Experimental Hematology, Vol. 24, No. 9, 01.12.1996.

Research output: Contribution to journalArticle

@article{1264fbc46d0c44588f698dde34753f5f,
title = "Administration of gm-csf to breast cancer patients mobilizes low numbers of tumor cells into leukapheresis harvests",
abstract = "Brugger et al (1994) reported that the administration of chemotherapy followed by G-CSF to patients (pts) with breast and lung cancer caused epithelial cells to appear in the circulation at a time dependent on whether bone marrow was involved with tumor. In this circumstance, it is, unclear if vascular damage caused by the chemotherapy contributes to the release of tumor cells. Consequently, we have evaluated mobilization of breast cancer cells into apheresis harvests in 15 pts with metastatic breast cancer following administration of GM-CSF alone. The tumor cells were detected by both immunocytochemistry(ICC) and RT-PCR to cytokeratins 8, 18 and 19. Blood and bone marrow were sampled prior to apheresis harvests and during cytokine administration, as well as, the pooled frozen sample administered to the patient and a blood sample immediately post-transplant. We observed positive apheresis harvests in pts with negative blood and marrow prior to mobilization. These results demonstrate that breast cancer cells are mobilized in low numbers, into leukapheresis harvests following the administration of cytokine alone and some tumor cells are likely to be re-infused with the harvest. However, the clinical significance of reinfusion of small numbers of mobilized tumor cells remains to be determined.",
author = "S. Tarantolo and M. Travstman and Reed, {Elizabeth Cecile} and B. Murohv and S. Mann and A. Kessinqer and Sharp, {John G}",
year = "1996",
month = "12",
day = "1",
language = "English (US)",
volume = "24",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - Administration of gm-csf to breast cancer patients mobilizes low numbers of tumor cells into leukapheresis harvests

AU - Tarantolo, S.

AU - Travstman, M.

AU - Reed, Elizabeth Cecile

AU - Murohv, B.

AU - Mann, S.

AU - Kessinqer, A.

AU - Sharp, John G

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Brugger et al (1994) reported that the administration of chemotherapy followed by G-CSF to patients (pts) with breast and lung cancer caused epithelial cells to appear in the circulation at a time dependent on whether bone marrow was involved with tumor. In this circumstance, it is, unclear if vascular damage caused by the chemotherapy contributes to the release of tumor cells. Consequently, we have evaluated mobilization of breast cancer cells into apheresis harvests in 15 pts with metastatic breast cancer following administration of GM-CSF alone. The tumor cells were detected by both immunocytochemistry(ICC) and RT-PCR to cytokeratins 8, 18 and 19. Blood and bone marrow were sampled prior to apheresis harvests and during cytokine administration, as well as, the pooled frozen sample administered to the patient and a blood sample immediately post-transplant. We observed positive apheresis harvests in pts with negative blood and marrow prior to mobilization. These results demonstrate that breast cancer cells are mobilized in low numbers, into leukapheresis harvests following the administration of cytokine alone and some tumor cells are likely to be re-infused with the harvest. However, the clinical significance of reinfusion of small numbers of mobilized tumor cells remains to be determined.

AB - Brugger et al (1994) reported that the administration of chemotherapy followed by G-CSF to patients (pts) with breast and lung cancer caused epithelial cells to appear in the circulation at a time dependent on whether bone marrow was involved with tumor. In this circumstance, it is, unclear if vascular damage caused by the chemotherapy contributes to the release of tumor cells. Consequently, we have evaluated mobilization of breast cancer cells into apheresis harvests in 15 pts with metastatic breast cancer following administration of GM-CSF alone. The tumor cells were detected by both immunocytochemistry(ICC) and RT-PCR to cytokeratins 8, 18 and 19. Blood and bone marrow were sampled prior to apheresis harvests and during cytokine administration, as well as, the pooled frozen sample administered to the patient and a blood sample immediately post-transplant. We observed positive apheresis harvests in pts with negative blood and marrow prior to mobilization. These results demonstrate that breast cancer cells are mobilized in low numbers, into leukapheresis harvests following the administration of cytokine alone and some tumor cells are likely to be re-infused with the harvest. However, the clinical significance of reinfusion of small numbers of mobilized tumor cells remains to be determined.

UR - http://www.scopus.com/inward/record.url?scp=3042945361&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042945361&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:3042945361

VL - 24

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 9

ER -