Adherence inhibition of enteropathogenic escherichia coli by chitooligosaccharides with specific degrees of acetylation and polymerization

Maria I. Quintero-Villegas, Berit B. Aam, John Rupnow, Morten Sørlie, Vincent G.H. Eijsink, Robert W. Hutkins

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Some oligosaccharides are known to act as molecular decoys by inhibiting pathogen adherence to epithelial cells. The present study was aimed at analyzing whether chitooligosaccharides (CHOS), that is, oligomers of d-glucosamine and N-acetyl-d-glucosamine, have such antiadherence activity. CHOS of varied degree of polymerization (DP) and fraction of acetylation (FA) were produced. Adherence of enteropathogenic Escherichia coli (EPEC) to the surface of a human HEp-2 cell line was determined in the absence or presence of the various CHOS fractions. Adherence was assessed by microscopic counting and image analysis of bacterial clusters and cells. The results showed that all CHOS fractions inhibited adherence of EPEC to HEp-2 cells. Hydrolysates with lower FA were more effective at reducing adherence. This effect is greater than that obtained with other oligosaccharides, such as galactooligosaccharides, applied at the same concentrations.

Original languageEnglish (US)
Pages (from-to)2748-2754
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number11
DOIs
StatePublished - Mar 20 2013

Fingerprint

chitooligosaccharides
Enteropathogenic Escherichia coli
enteropathogenic Escherichia coli
Acetylation
acetylation
Polymerization
polymerization
Escherichia coli
glucosamine
Glucosamine
Oligosaccharides
oligosaccharides
galactooligosaccharides
Pathogens
hydrolysates
Oligomers
Image analysis
Cluster Analysis
epithelial cells
Epithelial Cells

Keywords

  • adherence
  • chitinase
  • chitooligosaccharides
  • chitosan
  • chitosanase
  • oligosaccharides

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Adherence inhibition of enteropathogenic escherichia coli by chitooligosaccharides with specific degrees of acetylation and polymerization. / Quintero-Villegas, Maria I.; Aam, Berit B.; Rupnow, John; Sørlie, Morten; Eijsink, Vincent G.H.; Hutkins, Robert W.

In: Journal of Agricultural and Food Chemistry, Vol. 61, No. 11, 20.03.2013, p. 2748-2754.

Research output: Contribution to journalArticle

Quintero-Villegas, Maria I. ; Aam, Berit B. ; Rupnow, John ; Sørlie, Morten ; Eijsink, Vincent G.H. ; Hutkins, Robert W. / Adherence inhibition of enteropathogenic escherichia coli by chitooligosaccharides with specific degrees of acetylation and polymerization. In: Journal of Agricultural and Food Chemistry. 2013 ; Vol. 61, No. 11. pp. 2748-2754.
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AU - Sørlie, Morten

AU - Eijsink, Vincent G.H.

AU - Hutkins, Robert W.

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