Adherence inhibition of enteric pathogens to epithelial cells by bovine colostrum fractions

Maria X. Maldonado-Gomez, Hyeyoung Lee, Daniela Barile, Mei Lu, Robert W. Hutkins

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

To initiate colonization, microbial pathogens express adhesins that recognize and adhere to host tissue cells. One strategy for inhibiting adherence relies on the presence of molecules that mimic epithelial receptor sites. In this study, invitro experiments using HEp-2cells were performed to assess the anti-adherence properties of bovine colostrum fractions against enteropathogenic Escherichia coli, Cronobacter sakazakii, and Salmonella enterica serovar Typhymurium. The results showed that protein-reduced colostrum significantly inhibited binding (from 68 to 99%) of all three pathogens to tissue culture cells. Ultrafiltration (<10,000Da) and nanofiltration (<1000Da) permeates were also effective, with the ultrafiltration permeate having the broadest activity. In contrast, the nanofiltration retentate was less effective since it inhibited only Salmonella. Mass spectrometry revealed minor differences in the oligosaccharide profile, with the acidic oligosaccharides being the predominant species. This study provides evidence that bovine colostrum contains a heterogeneous mixture of anti-adherence components.

Original languageEnglish (US)
Pages (from-to)24-32
Number of pages9
JournalInternational Dairy Journal
Volume40
DOIs
StatePublished - Jan 1 2015

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ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology

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