Adherence inhibition of enteric pathogens to epithelial cells by bovine colostrum fractions

Maria X. Maldonado-Gomez, Hyeyoung Lee, Daniela Barile, Mei Lu, Robert W. Hutkins

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To initiate colonization, microbial pathogens express adhesins that recognize and adhere to host tissue cells. One strategy for inhibiting adherence relies on the presence of molecules that mimic epithelial receptor sites. In this study, invitro experiments using HEp-2cells were performed to assess the anti-adherence properties of bovine colostrum fractions against enteropathogenic Escherichia coli, Cronobacter sakazakii, and Salmonella enterica serovar Typhymurium. The results showed that protein-reduced colostrum significantly inhibited binding (from 68 to 99%) of all three pathogens to tissue culture cells. Ultrafiltration (<10,000Da) and nanofiltration (<1000Da) permeates were also effective, with the ultrafiltration permeate having the broadest activity. In contrast, the nanofiltration retentate was less effective since it inhibited only Salmonella. Mass spectrometry revealed minor differences in the oligosaccharide profile, with the acidic oligosaccharides being the predominant species. This study provides evidence that bovine colostrum contains a heterogeneous mixture of anti-adherence components.

Original languageEnglish (US)
Pages (from-to)24-32
Number of pages9
JournalInternational Dairy Journal
Volume40
DOIs
StatePublished - Jan 1 2015

Fingerprint

cow colostrum
Colostrum
permeates
ultrafiltration
oligosaccharides
epithelial cells
Epithelial Cells
Ultrafiltration
microbial colonization
Cronobacter sakazakii
Oligosaccharides
enteropathogenic Escherichia coli
adhesins
pathogens
Salmonella enterica
colostrum
tissue culture
Enteropathogenic Escherichia coli
Salmonella
serotypes

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology

Cite this

Adherence inhibition of enteric pathogens to epithelial cells by bovine colostrum fractions. / Maldonado-Gomez, Maria X.; Lee, Hyeyoung; Barile, Daniela; Lu, Mei; Hutkins, Robert W.

In: International Dairy Journal, Vol. 40, 01.01.2015, p. 24-32.

Research output: Contribution to journalArticle

Maldonado-Gomez, Maria X. ; Lee, Hyeyoung ; Barile, Daniela ; Lu, Mei ; Hutkins, Robert W. / Adherence inhibition of enteric pathogens to epithelial cells by bovine colostrum fractions. In: International Dairy Journal. 2015 ; Vol. 40. pp. 24-32.
@article{261e61406cfe49af9cd748be6e91c024,
title = "Adherence inhibition of enteric pathogens to epithelial cells by bovine colostrum fractions",
abstract = "To initiate colonization, microbial pathogens express adhesins that recognize and adhere to host tissue cells. One strategy for inhibiting adherence relies on the presence of molecules that mimic epithelial receptor sites. In this study, invitro experiments using HEp-2cells were performed to assess the anti-adherence properties of bovine colostrum fractions against enteropathogenic Escherichia coli, Cronobacter sakazakii, and Salmonella enterica serovar Typhymurium. The results showed that protein-reduced colostrum significantly inhibited binding (from 68 to 99{\%}) of all three pathogens to tissue culture cells. Ultrafiltration (<10,000Da) and nanofiltration (<1000Da) permeates were also effective, with the ultrafiltration permeate having the broadest activity. In contrast, the nanofiltration retentate was less effective since it inhibited only Salmonella. Mass spectrometry revealed minor differences in the oligosaccharide profile, with the acidic oligosaccharides being the predominant species. This study provides evidence that bovine colostrum contains a heterogeneous mixture of anti-adherence components.",
author = "Maldonado-Gomez, {Maria X.} and Hyeyoung Lee and Daniela Barile and Mei Lu and Hutkins, {Robert W.}",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.idairyj.2014.08.014",
language = "English (US)",
volume = "40",
pages = "24--32",
journal = "International Dairy Journal",
issn = "0958-6946",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Adherence inhibition of enteric pathogens to epithelial cells by bovine colostrum fractions

AU - Maldonado-Gomez, Maria X.

AU - Lee, Hyeyoung

AU - Barile, Daniela

AU - Lu, Mei

AU - Hutkins, Robert W.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - To initiate colonization, microbial pathogens express adhesins that recognize and adhere to host tissue cells. One strategy for inhibiting adherence relies on the presence of molecules that mimic epithelial receptor sites. In this study, invitro experiments using HEp-2cells were performed to assess the anti-adherence properties of bovine colostrum fractions against enteropathogenic Escherichia coli, Cronobacter sakazakii, and Salmonella enterica serovar Typhymurium. The results showed that protein-reduced colostrum significantly inhibited binding (from 68 to 99%) of all three pathogens to tissue culture cells. Ultrafiltration (<10,000Da) and nanofiltration (<1000Da) permeates were also effective, with the ultrafiltration permeate having the broadest activity. In contrast, the nanofiltration retentate was less effective since it inhibited only Salmonella. Mass spectrometry revealed minor differences in the oligosaccharide profile, with the acidic oligosaccharides being the predominant species. This study provides evidence that bovine colostrum contains a heterogeneous mixture of anti-adherence components.

AB - To initiate colonization, microbial pathogens express adhesins that recognize and adhere to host tissue cells. One strategy for inhibiting adherence relies on the presence of molecules that mimic epithelial receptor sites. In this study, invitro experiments using HEp-2cells were performed to assess the anti-adherence properties of bovine colostrum fractions against enteropathogenic Escherichia coli, Cronobacter sakazakii, and Salmonella enterica serovar Typhymurium. The results showed that protein-reduced colostrum significantly inhibited binding (from 68 to 99%) of all three pathogens to tissue culture cells. Ultrafiltration (<10,000Da) and nanofiltration (<1000Da) permeates were also effective, with the ultrafiltration permeate having the broadest activity. In contrast, the nanofiltration retentate was less effective since it inhibited only Salmonella. Mass spectrometry revealed minor differences in the oligosaccharide profile, with the acidic oligosaccharides being the predominant species. This study provides evidence that bovine colostrum contains a heterogeneous mixture of anti-adherence components.

UR - http://www.scopus.com/inward/record.url?scp=84908316108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908316108&partnerID=8YFLogxK

U2 - 10.1016/j.idairyj.2014.08.014

DO - 10.1016/j.idairyj.2014.08.014

M3 - Article

AN - SCOPUS:84908316108

VL - 40

SP - 24

EP - 32

JO - International Dairy Journal

JF - International Dairy Journal

SN - 0958-6946

ER -