Adherence inhibition of cronobacter sakazakii to intestinal epithelial cells by prebiotic oligosaccharides

Maria Quintero, Maria Maldonado, Mariaelisa Perez-Munoz, Roberto Jimenez, Terry Fangman, John Rupnow, Anja Wittke, Michael Russell, Robert W Hutkins

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Cronobacter sakazakii is an opportunistic pathogen that has been implicated in meningitis, NEC, and sepsis in neonates. Colonization and subsequent infection and invasion of C. sakazakii require that the organism adheres to host cell surfaces. Agents that inhibit or block attachment of the pathogen to epithelial cells could be useful in reducing infections. The goal of this research was to assess the ability of prebiotic galactooligosaccharides (GOS) and polydextrose (PDX) to inhibit adherence of C. sakazakii 4603 to a HEp-2 human cell line. Adherence experiments were performed in the presence or absence of prebiotics using HEp-2 cells grown to confluency on glass coverslips. Prebiotics and bacteria were added and incubated for 3 h. Coverslips were washed, and adherence was determined by cultural and microscopic methods. When measured microscopically or by cultural methods, significant reductions in adherence (56 and 71%, respectively) of C. sakazakii were observed in the presence of GOS (16 mg/ml). Adherence inhibition also occurred (48%) when a GOS-PDX blend (8 mg/ml each) was tested, although PDX by itself had less effect. Similar results were also observed for Caco-2 cells and also for another strain of C. sakazakii (29004). These results suggest that GOS and PDX, alone and in combination, may have an anti-adhesive effect on C. sakazakii and directly inhibit the adherence to gastrointestinal epithelial cells.

Original languageEnglish (US)
Pages (from-to)1448-1454
Number of pages7
JournalCurrent Microbiology
Volume62
Issue number5
DOIs
StatePublished - May 1 2011

Fingerprint

polydextrose
Cronobacter sakazakii
Prebiotics
Oligosaccharides
Epithelial Cells
Caco-2 Cells
Infection
Meningitis
Adhesives
Glass
Sepsis
Bacteria
Cell Line
Research

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Adherence inhibition of cronobacter sakazakii to intestinal epithelial cells by prebiotic oligosaccharides. / Quintero, Maria; Maldonado, Maria; Perez-Munoz, Mariaelisa; Jimenez, Roberto; Fangman, Terry; Rupnow, John; Wittke, Anja; Russell, Michael; Hutkins, Robert W.

In: Current Microbiology, Vol. 62, No. 5, 01.05.2011, p. 1448-1454.

Research output: Contribution to journalArticle

Quintero, M, Maldonado, M, Perez-Munoz, M, Jimenez, R, Fangman, T, Rupnow, J, Wittke, A, Russell, M & Hutkins, RW 2011, 'Adherence inhibition of cronobacter sakazakii to intestinal epithelial cells by prebiotic oligosaccharides', Current Microbiology, vol. 62, no. 5, pp. 1448-1454. https://doi.org/10.1007/s00284-011-9882-8
Quintero, Maria ; Maldonado, Maria ; Perez-Munoz, Mariaelisa ; Jimenez, Roberto ; Fangman, Terry ; Rupnow, John ; Wittke, Anja ; Russell, Michael ; Hutkins, Robert W. / Adherence inhibition of cronobacter sakazakii to intestinal epithelial cells by prebiotic oligosaccharides. In: Current Microbiology. 2011 ; Vol. 62, No. 5. pp. 1448-1454.
@article{153b34cfd91444bcbf02185894b5bebd,
title = "Adherence inhibition of cronobacter sakazakii to intestinal epithelial cells by prebiotic oligosaccharides",
abstract = "Cronobacter sakazakii is an opportunistic pathogen that has been implicated in meningitis, NEC, and sepsis in neonates. Colonization and subsequent infection and invasion of C. sakazakii require that the organism adheres to host cell surfaces. Agents that inhibit or block attachment of the pathogen to epithelial cells could be useful in reducing infections. The goal of this research was to assess the ability of prebiotic galactooligosaccharides (GOS) and polydextrose (PDX) to inhibit adherence of C. sakazakii 4603 to a HEp-2 human cell line. Adherence experiments were performed in the presence or absence of prebiotics using HEp-2 cells grown to confluency on glass coverslips. Prebiotics and bacteria were added and incubated for 3 h. Coverslips were washed, and adherence was determined by cultural and microscopic methods. When measured microscopically or by cultural methods, significant reductions in adherence (56 and 71{\%}, respectively) of C. sakazakii were observed in the presence of GOS (16 mg/ml). Adherence inhibition also occurred (48{\%}) when a GOS-PDX blend (8 mg/ml each) was tested, although PDX by itself had less effect. Similar results were also observed for Caco-2 cells and also for another strain of C. sakazakii (29004). These results suggest that GOS and PDX, alone and in combination, may have an anti-adhesive effect on C. sakazakii and directly inhibit the adherence to gastrointestinal epithelial cells.",
author = "Maria Quintero and Maria Maldonado and Mariaelisa Perez-Munoz and Roberto Jimenez and Terry Fangman and John Rupnow and Anja Wittke and Michael Russell and Hutkins, {Robert W}",
year = "2011",
month = "5",
day = "1",
doi = "10.1007/s00284-011-9882-8",
language = "English (US)",
volume = "62",
pages = "1448--1454",
journal = "Current Microbiology",
issn = "0343-8651",
publisher = "Springer New York",
number = "5",

}

TY - JOUR

T1 - Adherence inhibition of cronobacter sakazakii to intestinal epithelial cells by prebiotic oligosaccharides

AU - Quintero, Maria

AU - Maldonado, Maria

AU - Perez-Munoz, Mariaelisa

AU - Jimenez, Roberto

AU - Fangman, Terry

AU - Rupnow, John

AU - Wittke, Anja

AU - Russell, Michael

AU - Hutkins, Robert W

PY - 2011/5/1

Y1 - 2011/5/1

N2 - Cronobacter sakazakii is an opportunistic pathogen that has been implicated in meningitis, NEC, and sepsis in neonates. Colonization and subsequent infection and invasion of C. sakazakii require that the organism adheres to host cell surfaces. Agents that inhibit or block attachment of the pathogen to epithelial cells could be useful in reducing infections. The goal of this research was to assess the ability of prebiotic galactooligosaccharides (GOS) and polydextrose (PDX) to inhibit adherence of C. sakazakii 4603 to a HEp-2 human cell line. Adherence experiments were performed in the presence or absence of prebiotics using HEp-2 cells grown to confluency on glass coverslips. Prebiotics and bacteria were added and incubated for 3 h. Coverslips were washed, and adherence was determined by cultural and microscopic methods. When measured microscopically or by cultural methods, significant reductions in adherence (56 and 71%, respectively) of C. sakazakii were observed in the presence of GOS (16 mg/ml). Adherence inhibition also occurred (48%) when a GOS-PDX blend (8 mg/ml each) was tested, although PDX by itself had less effect. Similar results were also observed for Caco-2 cells and also for another strain of C. sakazakii (29004). These results suggest that GOS and PDX, alone and in combination, may have an anti-adhesive effect on C. sakazakii and directly inhibit the adherence to gastrointestinal epithelial cells.

AB - Cronobacter sakazakii is an opportunistic pathogen that has been implicated in meningitis, NEC, and sepsis in neonates. Colonization and subsequent infection and invasion of C. sakazakii require that the organism adheres to host cell surfaces. Agents that inhibit or block attachment of the pathogen to epithelial cells could be useful in reducing infections. The goal of this research was to assess the ability of prebiotic galactooligosaccharides (GOS) and polydextrose (PDX) to inhibit adherence of C. sakazakii 4603 to a HEp-2 human cell line. Adherence experiments were performed in the presence or absence of prebiotics using HEp-2 cells grown to confluency on glass coverslips. Prebiotics and bacteria were added and incubated for 3 h. Coverslips were washed, and adherence was determined by cultural and microscopic methods. When measured microscopically or by cultural methods, significant reductions in adherence (56 and 71%, respectively) of C. sakazakii were observed in the presence of GOS (16 mg/ml). Adherence inhibition also occurred (48%) when a GOS-PDX blend (8 mg/ml each) was tested, although PDX by itself had less effect. Similar results were also observed for Caco-2 cells and also for another strain of C. sakazakii (29004). These results suggest that GOS and PDX, alone and in combination, may have an anti-adhesive effect on C. sakazakii and directly inhibit the adherence to gastrointestinal epithelial cells.

UR - http://www.scopus.com/inward/record.url?scp=79954419203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79954419203&partnerID=8YFLogxK

U2 - 10.1007/s00284-011-9882-8

DO - 10.1007/s00284-011-9882-8

M3 - Article

VL - 62

SP - 1448

EP - 1454

JO - Current Microbiology

JF - Current Microbiology

SN - 0343-8651

IS - 5

ER -