Adenovirus type-2 in a patient with lethal hemorrhagic colonic ulcers and chronic active Epstein-Barr virus infection

M. Okano, Geoffrey Milton Thiele, J. R. Davis, W. M. Nauseef, F. Mitros, D. T. Purtilo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

An adenovirus type-2 was isolated from peripheral blood lymphocytes and throat washings from a patient with severe chronic active Epstein-Barr virus infection. Despite the Epstein-Barr virus reactivation, attempts to establish spontaneous lymphoblastoid cell lines from peripheral blood lymphocytes and to immortalize cord lymphocytes with throat washings were unsuccessful due to a marked cytopathic effect. The supernatants from the cultures induced cytopathic effect in cultured cord lymphocytes, MRC-5 cells, A-549 cells, or Vero cells. Virus particles with adenovirus morphology were seen by electron microscopy. Using type-specific antisera, the isolate was identified as adenovirus type-2. In addition, both Epstein-Barr virus and adenovirus type-2 genomes were seen in the colonic tissues and spleen. These results suggest that the combination of Epstein-Barr virus and adenovirus type-2 may be etiologic agents in the development of chronic active Epstein-Barr virus infection in this patient.

Original languageEnglish (US)
Pages (from-to)693-699
Number of pages7
JournalAnnals of internal medicine
Volume108
Issue number5
DOIs
StatePublished - Jan 1 1988

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Epstein-Barr Virus Infections
Adenoviridae
Ulcer
Human Herpesvirus 4
Lymphocytes
Pharynx
Vero Cells
Virion
Immune Sera
Electron Microscopy
Spleen
Genome
Cell Line

ASJC Scopus subject areas

  • Internal Medicine

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Adenovirus type-2 in a patient with lethal hemorrhagic colonic ulcers and chronic active Epstein-Barr virus infection. / Okano, M.; Thiele, Geoffrey Milton; Davis, J. R.; Nauseef, W. M.; Mitros, F.; Purtilo, D. T.

In: Annals of internal medicine, Vol. 108, No. 5, 01.01.1988, p. 693-699.

Research output: Contribution to journalArticle

Okano, M. ; Thiele, Geoffrey Milton ; Davis, J. R. ; Nauseef, W. M. ; Mitros, F. ; Purtilo, D. T. / Adenovirus type-2 in a patient with lethal hemorrhagic colonic ulcers and chronic active Epstein-Barr virus infection. In: Annals of internal medicine. 1988 ; Vol. 108, No. 5. pp. 693-699.
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