Adenosine uptake sites in rat brain: Identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase

J. I. Nagy, J. D. Geiger, P. E. Daddona

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalNeuroscience Letters
Volume55
Issue number1
DOIs
StatePublished - Mar 22 1985

Fingerprint

Adenosine Deaminase
Adenosine
Brain
Nucleosides
Ligands
Neurons
Membranes

Keywords

  • [H]nitrobenzylthioinosine
  • adenosine
  • autoradiography
  • deaminase
  • immunohistochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Adenosine uptake sites in rat brain : Identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase. / Nagy, J. I.; Geiger, J. D.; Daddona, P. E.

In: Neuroscience Letters, Vol. 55, No. 1, 22.03.1985, p. 47-53.

Research output: Contribution to journalArticle

@article{5f98a88a92a943478e20f7d552dea364,
title = "Adenosine uptake sites in rat brain: Identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase",
abstract = "The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.",
keywords = "[H]nitrobenzylthioinosine, adenosine, autoradiography, deaminase, immunohistochemistry",
author = "Nagy, {J. I.} and Geiger, {J. D.} and Daddona, {P. E.}",
year = "1985",
month = "3",
day = "22",
doi = "10.1016/0304-3940(85)90310-6",
language = "English (US)",
volume = "55",
pages = "47--53",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Adenosine uptake sites in rat brain

T2 - Identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase

AU - Nagy, J. I.

AU - Geiger, J. D.

AU - Daddona, P. E.

PY - 1985/3/22

Y1 - 1985/3/22

N2 - The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.

AB - The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sites are heterogeneously distributed and that there is an exact correspondence between areas containing [3H]NBI sites and ADA-immunoreactive neurons. Our results indicate that [3H]NBI and ADA are potential markers for revealing anatomical sites at which actions of adenosine may be expressed.

KW - [H]nitrobenzylthioinosine

KW - adenosine

KW - autoradiography

KW - deaminase

KW - immunohistochemistry

UR - http://www.scopus.com/inward/record.url?scp=0021954130&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021954130&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(85)90310-6

DO - 10.1016/0304-3940(85)90310-6

M3 - Article

C2 - 3991077

AN - SCOPUS:0021954130

VL - 55

SP - 47

EP - 53

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -