Adenomatous polyposis coli regulates axon arborization and cytoskeleton organization via its n-terminus

Youjun Chen, Xu Tian, Woo Yang Kim, William D. Snider

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Conditional deletion of APC leads to marked disruption of cortical development and to excessive axonal branching of cortical neurons. However, little is known about the cell biological basis of this neuronal morphological regulation. Here we show that APC deficient cortical neuronal growth cones exhibit marked disruption of both microtubule and actin cytoskeleton. Functional analysis of the different APC domains revealed that axonal branches do not result from stabilized β-catenin, and that the C-terminus of APC containing microtubule regulatory domains only partially rescues the branching phenotype. Surprisingly, the N-terminus of APC containing the oligomerization domain and the armadillo repeats completely rescues the branching and cytoskeletal abnormalities. Our data indicate that APC is required for appropriate axon morphological development and that the N-terminus of APC is important for regulation of the neuronal cytoskeleton.

Original languageEnglish (US)
Article numbere24335
JournalPloS one
Volume6
Issue number9
DOIs
StatePublished - Sep 12 2011

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Catenins
Oligomerization
Functional analysis
Adenomatous Polyposis Coli
cytoskeleton
Cytoskeleton
axons
Microtubules
Neurons
Axons
branching
Cones
Actins
Armadillos
Growth Cones
microtubules
Actin Cytoskeleton
Dasypodidae
cones (retina)
microfilaments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Adenomatous polyposis coli regulates axon arborization and cytoskeleton organization via its n-terminus. / Chen, Youjun; Tian, Xu; Kim, Woo Yang; Snider, William D.

In: PloS one, Vol. 6, No. 9, e24335, 12.09.2011.

Research output: Contribution to journalArticle

Chen, Youjun ; Tian, Xu ; Kim, Woo Yang ; Snider, William D. / Adenomatous polyposis coli regulates axon arborization and cytoskeleton organization via its n-terminus. In: PloS one. 2011 ; Vol. 6, No. 9.
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