Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage

Toshihiko Fukuda, Kaustav Majumder, Hua Zhang, Toshiro Matsui, Yoshinori Mine

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

It has previously demonstrated that adenine (6-amino-6H-purine) exhibited a vasoactive effect in contracted aorta. The adenine receptor (AdeR) has recently been identified as a G protein coupled purinergic receptor in diverse organs. It has been suggested that adenine acts as a signaling molecule through the activation of AdeR. The present study investigated the effect of adenine on endotoxin-stimulated mouse macrophages (RAW 264.7 cells) and focused on the AdeR signaling pathway. RAW 264.7 cells were treated with adenine and stimulated with lipopolysaccharide (LPS, Toll-like receptor 4 agonist) from Escherichia coli. Adenine inhibited tumor necrosis factor (TNF)-α secretion in RAW 264.7 cells. The inhibition by adenine was significantly attenuated in the presence of either adenyl cyclase (AC) or protein kinase A (PKA) inhibitors. Furthermore, adenine restored AdeR gene expression. These results firstly demonstrated that adenine acts as an anti-endotoxin compounds through a AdeR/AC/PKA signaling cascade.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalJournal of Functional Foods
Volume28
DOIs
StatePublished - Jan 1 2017

Fingerprint

Adenine
adenine
anti-inflammatory activity
macrophages
Anti-Inflammatory Agents
Macrophages
receptors
mice
adenylate cyclase
cAMP-dependent protein kinase
endotoxins
Cyclic AMP-Dependent Protein Kinases
Adenylyl Cyclases
Endotoxins
Purinergic Receptors
Toll-Like Receptor 4
tumor necrosis factors
cells
Protein Kinase Inhibitors
purines

Keywords

  • Adenine
  • Adenine receptor
  • Anti-inflammation
  • LPS
  • Macrophage

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage. / Fukuda, Toshihiko; Majumder, Kaustav; Zhang, Hua; Matsui, Toshiro; Mine, Yoshinori.

In: Journal of Functional Foods, Vol. 28, 01.01.2017, p. 235-239.

Research output: Contribution to journalArticle

Fukuda, Toshihiko ; Majumder, Kaustav ; Zhang, Hua ; Matsui, Toshiro ; Mine, Yoshinori. / Adenine has an anti-inflammatory effect through the activation of adenine receptor signaling in mouse macrophage. In: Journal of Functional Foods. 2017 ; Vol. 28. pp. 235-239.
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