Addition of an α-hydroxy acid to the genetic code of bacteria

Jiantao Guo, Jiangyun Wang, J. Christopher Anderson, Peter G. Schultz

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

(Chemical Equation Presented) Playing tag: The α-hydroxy acid p-hydroxy-L-phenyllactic acid has been genetically incorporated into proteins in E. coli in response to an amber nonsense codon. The site-specific introduction of backbone ester mutations was used for the site-specific hydrolysis of an affinity tag, as well as for determination of the energetic contributions of backbone hydrogen bonds to protein stability.

Original languageEnglish (US)
Pages (from-to)722-725
Number of pages4
JournalAngewandte Chemie - International Edition
Volume47
Issue number4
DOIs
StatePublished - Jan 24 2008

Fingerprint

Hydroxy Acids
Bacteria
Amber
Proteins
Acids
Escherichia coli
Hydrolysis
Esters
Hydrogen bonds

Keywords

  • Backbone mutations
  • Biosynthesis
  • Proteins
  • α-hydroxy acids

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Addition of an α-hydroxy acid to the genetic code of bacteria. / Guo, Jiantao; Wang, Jiangyun; Anderson, J. Christopher; Schultz, Peter G.

In: Angewandte Chemie - International Edition, Vol. 47, No. 4, 24.01.2008, p. 722-725.

Research output: Contribution to journalArticle

Guo, Jiantao ; Wang, Jiangyun ; Anderson, J. Christopher ; Schultz, Peter G. / Addition of an α-hydroxy acid to the genetic code of bacteria. In: Angewandte Chemie - International Edition. 2008 ; Vol. 47, No. 4. pp. 722-725.
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