Adamantinoma-like Ewing's sarcoma

Genomic confirmation, phenotypic drift

Julia A. Bridge, Mary E. Fidler, James R. Neff, Joanne Degenhardt, Mei Wang, Craig Walker, Howard D. Dorfman, K. Scott Baker, Thomas A. Seemayer

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Ewing's sarcoma, a highly malignant neoplasm, is characterized by an 11;22 translocation [t(11 ;22) (q24;q12)], resulting in the fusion of genes FLII and EWS. Adamantinoma of extragnathic bones, a low-grade malignant neoplasm with epithelial features, is not typically considered in the differential diagnosis of Ewing's sarcoma. In this study, three osseous Ewing's sarcomas with histological, immunohistochemical, or ultrastructural epithelial features were subjected to reverse transcription-polymerase chain reaction and sequencing studies for the Ewing's sarcoma molecular rearrangement. (Two of the three cases were originally described as adamantinomas or nontypical Ewing's sarcoma before the availability of genetic characterization.) In addition, traditional cytogenetic analysis and a unique combined interphase molecular cytogenetic/ immunocytochemical approach with bicolor 11;22 translocation breakpoint flanking probes (cosmids) and pancytokeratin antibodies were performed on one neoplasm. A t(11;22) (q24;q12) was found in one neoplasm and a type II EWS/FLI-I fusion transcript was detected in all three neoplasms. The combined genetic/immunocytochemical approach revealed the presence of the 11;22 translocation in the nuclei of cytokeratin immunoreactive cells. These genotypic and phenotypic findings delineate a novel Ewing's sarcoma histologic variant, 'adamantinoma-like Ewing's sarcoma'.

Original languageEnglish (US)
Pages (from-to)159-165
Number of pages7
JournalAmerican Journal of Surgical Pathology
Volume23
Issue number2
DOIs
StatePublished - Feb 1 1999

Fingerprint

Adamantinoma
Ewing's Sarcoma
Neoplasms
Cosmids
Cytogenetic Analysis
Gene Fusion
Interphase
Keratins
Cytogenetics
Reverse Transcription
Differential Diagnosis
Carcinoma
Bone and Bones
Polymerase Chain Reaction
Antibodies

Keywords

  • Adamantinoma
  • EWS/FLI1
  • Ewing's sarcoma
  • Karyotype
  • RT/PCR

ASJC Scopus subject areas

  • Anatomy
  • Surgery
  • Pathology and Forensic Medicine

Cite this

Bridge, J. A., Fidler, M. E., Neff, J. R., Degenhardt, J., Wang, M., Walker, C., ... Seemayer, T. A. (1999). Adamantinoma-like Ewing's sarcoma: Genomic confirmation, phenotypic drift. American Journal of Surgical Pathology, 23(2), 159-165. https://doi.org/10.1097/00000478-199902000-00004

Adamantinoma-like Ewing's sarcoma : Genomic confirmation, phenotypic drift. / Bridge, Julia A.; Fidler, Mary E.; Neff, James R.; Degenhardt, Joanne; Wang, Mei; Walker, Craig; Dorfman, Howard D.; Baker, K. Scott; Seemayer, Thomas A.

In: American Journal of Surgical Pathology, Vol. 23, No. 2, 01.02.1999, p. 159-165.

Research output: Contribution to journalArticle

Bridge, JA, Fidler, ME, Neff, JR, Degenhardt, J, Wang, M, Walker, C, Dorfman, HD, Baker, KS & Seemayer, TA 1999, 'Adamantinoma-like Ewing's sarcoma: Genomic confirmation, phenotypic drift', American Journal of Surgical Pathology, vol. 23, no. 2, pp. 159-165. https://doi.org/10.1097/00000478-199902000-00004
Bridge, Julia A. ; Fidler, Mary E. ; Neff, James R. ; Degenhardt, Joanne ; Wang, Mei ; Walker, Craig ; Dorfman, Howard D. ; Baker, K. Scott ; Seemayer, Thomas A. / Adamantinoma-like Ewing's sarcoma : Genomic confirmation, phenotypic drift. In: American Journal of Surgical Pathology. 1999 ; Vol. 23, No. 2. pp. 159-165.
@article{91ecc1fccead43f7ae313dc92704ffb1,
title = "Adamantinoma-like Ewing's sarcoma: Genomic confirmation, phenotypic drift",
abstract = "Ewing's sarcoma, a highly malignant neoplasm, is characterized by an 11;22 translocation [t(11 ;22) (q24;q12)], resulting in the fusion of genes FLII and EWS. Adamantinoma of extragnathic bones, a low-grade malignant neoplasm with epithelial features, is not typically considered in the differential diagnosis of Ewing's sarcoma. In this study, three osseous Ewing's sarcomas with histological, immunohistochemical, or ultrastructural epithelial features were subjected to reverse transcription-polymerase chain reaction and sequencing studies for the Ewing's sarcoma molecular rearrangement. (Two of the three cases were originally described as adamantinomas or nontypical Ewing's sarcoma before the availability of genetic characterization.) In addition, traditional cytogenetic analysis and a unique combined interphase molecular cytogenetic/ immunocytochemical approach with bicolor 11;22 translocation breakpoint flanking probes (cosmids) and pancytokeratin antibodies were performed on one neoplasm. A t(11;22) (q24;q12) was found in one neoplasm and a type II EWS/FLI-I fusion transcript was detected in all three neoplasms. The combined genetic/immunocytochemical approach revealed the presence of the 11;22 translocation in the nuclei of cytokeratin immunoreactive cells. These genotypic and phenotypic findings delineate a novel Ewing's sarcoma histologic variant, 'adamantinoma-like Ewing's sarcoma'.",
keywords = "Adamantinoma, EWS/FLI1, Ewing's sarcoma, Karyotype, RT/PCR",
author = "Bridge, {Julia A.} and Fidler, {Mary E.} and Neff, {James R.} and Joanne Degenhardt and Mei Wang and Craig Walker and Dorfman, {Howard D.} and Baker, {K. Scott} and Seemayer, {Thomas A.}",
year = "1999",
month = "2",
day = "1",
doi = "10.1097/00000478-199902000-00004",
language = "English (US)",
volume = "23",
pages = "159--165",
journal = "American Journal of Surgical Pathology",
issn = "0147-5185",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Adamantinoma-like Ewing's sarcoma

T2 - Genomic confirmation, phenotypic drift

AU - Bridge, Julia A.

AU - Fidler, Mary E.

AU - Neff, James R.

AU - Degenhardt, Joanne

AU - Wang, Mei

AU - Walker, Craig

AU - Dorfman, Howard D.

AU - Baker, K. Scott

AU - Seemayer, Thomas A.

PY - 1999/2/1

Y1 - 1999/2/1

N2 - Ewing's sarcoma, a highly malignant neoplasm, is characterized by an 11;22 translocation [t(11 ;22) (q24;q12)], resulting in the fusion of genes FLII and EWS. Adamantinoma of extragnathic bones, a low-grade malignant neoplasm with epithelial features, is not typically considered in the differential diagnosis of Ewing's sarcoma. In this study, three osseous Ewing's sarcomas with histological, immunohistochemical, or ultrastructural epithelial features were subjected to reverse transcription-polymerase chain reaction and sequencing studies for the Ewing's sarcoma molecular rearrangement. (Two of the three cases were originally described as adamantinomas or nontypical Ewing's sarcoma before the availability of genetic characterization.) In addition, traditional cytogenetic analysis and a unique combined interphase molecular cytogenetic/ immunocytochemical approach with bicolor 11;22 translocation breakpoint flanking probes (cosmids) and pancytokeratin antibodies were performed on one neoplasm. A t(11;22) (q24;q12) was found in one neoplasm and a type II EWS/FLI-I fusion transcript was detected in all three neoplasms. The combined genetic/immunocytochemical approach revealed the presence of the 11;22 translocation in the nuclei of cytokeratin immunoreactive cells. These genotypic and phenotypic findings delineate a novel Ewing's sarcoma histologic variant, 'adamantinoma-like Ewing's sarcoma'.

AB - Ewing's sarcoma, a highly malignant neoplasm, is characterized by an 11;22 translocation [t(11 ;22) (q24;q12)], resulting in the fusion of genes FLII and EWS. Adamantinoma of extragnathic bones, a low-grade malignant neoplasm with epithelial features, is not typically considered in the differential diagnosis of Ewing's sarcoma. In this study, three osseous Ewing's sarcomas with histological, immunohistochemical, or ultrastructural epithelial features were subjected to reverse transcription-polymerase chain reaction and sequencing studies for the Ewing's sarcoma molecular rearrangement. (Two of the three cases were originally described as adamantinomas or nontypical Ewing's sarcoma before the availability of genetic characterization.) In addition, traditional cytogenetic analysis and a unique combined interphase molecular cytogenetic/ immunocytochemical approach with bicolor 11;22 translocation breakpoint flanking probes (cosmids) and pancytokeratin antibodies were performed on one neoplasm. A t(11;22) (q24;q12) was found in one neoplasm and a type II EWS/FLI-I fusion transcript was detected in all three neoplasms. The combined genetic/immunocytochemical approach revealed the presence of the 11;22 translocation in the nuclei of cytokeratin immunoreactive cells. These genotypic and phenotypic findings delineate a novel Ewing's sarcoma histologic variant, 'adamantinoma-like Ewing's sarcoma'.

KW - Adamantinoma

KW - EWS/FLI1

KW - Ewing's sarcoma

KW - Karyotype

KW - RT/PCR

UR - http://www.scopus.com/inward/record.url?scp=0033014436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033014436&partnerID=8YFLogxK

U2 - 10.1097/00000478-199902000-00004

DO - 10.1097/00000478-199902000-00004

M3 - Article

VL - 23

SP - 159

EP - 165

JO - American Journal of Surgical Pathology

JF - American Journal of Surgical Pathology

SN - 0147-5185

IS - 2

ER -