Acute effects of phorbol esters on receptor-mediated IP3, cAMP, and progesterone levels in rat granulosa cells

J. S. Davis, L. L. Weakland, R. G. Coffey, L. A. West

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Luteinizing hormone (LH) stimulates the formation of adenosine 3',5'-cyclic monophosphate (cAMP) and inositol trisphosphate (IP3) in rat granulosa cells. This report describes the effects of protein kinase C activators on second messenger generation in isolated rat granulosa cells. The protein kinase C activator 12-O-tetradecanoylphorbol 13-acetate (TPA) completely inhibited LH-stimulated inositol phosphate accumulation. The inhibitory effects of TPA were rapid (5-15 min) and concentration dependent with 50 nM TPA producing maximally inhibitory effects. However, 30-min incubations with 10-100 nM TPA had no effect on LH-stimulated cAMP or progesterone levels. The inhibitory effect of TPA could not be overcome by high concentrations of LH. TPA also inhibited gonadotropin-releasing hormone-stimulated phospholipase C activity, although to a much lesser extent. Increased inositol phosphate degradation and reduced inositol phospholipid synthesis were unlikely explanations for the effects of TPA. The results indicate that phorbol esters modulate the inositol phospholipid-phospholipase C transmembrane signaling system in rat granulosa cells. The results suggest that phorbol esters may alter the coupling of the hormone receptor complex to phospholipase C.

Original languageEnglish (US)
Pages (from-to)19/3
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume256
Issue number3
StatePublished - Jan 1 1989

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Granulosa Cells
Phorbol Esters
Tetradecanoylphorbol Acetate
Progesterone
Rats
Acetates
Luteinizing Hormone
Type C Phospholipases
Inositol Phosphates
Phosphatidylinositols
Protein Kinase C
Second Messenger Systems
Inositol
phorbol ester receptor
Gonadotropin-Releasing Hormone
Cyclic AMP
Hormones
Degradation

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Acute effects of phorbol esters on receptor-mediated IP3, cAMP, and progesterone levels in rat granulosa cells. / Davis, J. S.; Weakland, L. L.; Coffey, R. G.; West, L. A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 256, No. 3, 01.01.1989, p. 19/3.

Research output: Contribution to journalArticle

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