Acute β - N -Methylamino-L-alanine Toxicity in a Mouse Model

Maitham Ahmed Al-Sammak, Douglas G. Rogers, Kyle D. Hoagland

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The cyanobacterial neurotoxin β-N-methylamino-L-alanine (BMAA) is considered to be an "excitotoxin," and its suggested mechanism of action is killing neurons. Long-term exposure to L-BMAA is believed to lead to neurodegenerative diseases including Parkinson's and Alzheimer's diseases and amyotrophic lateral sclerosis (Lou Gehrig's disease). Objectives of this study were to determine the presumptive median lethal dose (LD, the Lowest-Observed-Adverse-Effect Level (LOAEL), and histopathologic lesions caused by the naturally occurring BMAA isomer, L-BMAA, in mice. Seventy NIH Swiss Outbred mice (35 male and 35 female) were used. Treatment group mice were injected intraperitoneally with 0.03, 0.3, 1, 2, and 3 mg/g body weight L-BMAA, respectively, and control mice were sham-injected. The presumptive LDof L-BMAA was 3 mg/g BW and the LOAEL was 2 mg/g BW. There were no histopathologic lesions in brain, liver, heart, kidney, lung, or spleen in any of the mice during the 14-day study. L-BMAA was detected in brains and livers in all of treated mice but not in control mice. Males injected with 0.03 mg/g BW, 0.3 mg/g BW, and 3.0 mg/g BW L-BMAA showed consistently higher concentrations (P < 0.01) in brain and liver samples as compared to females in those respective groups.

Original languageEnglish (US)
Article number739746
JournalJournal of Toxicology
Volume2015
DOIs
StatePublished - Jan 1 2015

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Alanine
Toxicity
Liver
Brain
Neurotoxins
Amyotrophic Lateral Sclerosis
Neurodegenerative diseases
Isomers
Neurons
beta-N-methylamino-L-alanine
Neurodegenerative Diseases
Parkinson Disease
Alzheimer Disease
Spleen
Body Weight
Kidney
Lung

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

Cite this

Al-Sammak, M. A., Rogers, D. G., & Hoagland, K. D. (2015). Acute β - N -Methylamino-L-alanine Toxicity in a Mouse Model. Journal of Toxicology, 2015, [739746]. https://doi.org/10.1155/2015/739746

Acute β - N -Methylamino-L-alanine Toxicity in a Mouse Model. / Al-Sammak, Maitham Ahmed; Rogers, Douglas G.; Hoagland, Kyle D.

In: Journal of Toxicology, Vol. 2015, 739746, 01.01.2015.

Research output: Contribution to journalArticle

Al-Sammak, Maitham Ahmed ; Rogers, Douglas G. ; Hoagland, Kyle D. / Acute β - N -Methylamino-L-alanine Toxicity in a Mouse Model. In: Journal of Toxicology. 2015 ; Vol. 2015.
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