Activity of the Arabidopsis RD29A and RD29B promoter elements in soybean under water stress

Saadia Bihmidine, Jiusheng Lin, Julie M. Stone, Tala Awada, James E. Specht, Tom E. Clemente

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The constitutive and drought-induced activities of the Arabidopsis thaliana RD29A and RD29B promoters were monitored in soybean (Glycine max (L.) Merr.] via fusions with the visual marker gene β-glucuronidase (GUS). Physiological responses of soybean plants were monitored over 9 days of water deprivation under greenhouse conditions. Data were used to select appropriate time points to monitor the activities of the respective promoter elements. Qualitative and quantitative assays for GUS expression were conducted in root and leaf tissues, from plants under well-watered and dry-down conditions. Both RD29A and RD29B promoters were significantly activated in soybean plants subjected to dry-down conditions. However, a low level of constitutive promoter activity was also observed in both root and leaves of plants under well-watered conditions. GUS expression was notably higher in roots than in leaves. These observations suggest that the respective drought-responsive regulatory elements present in the RD29X promoters may be useful in controlling targeted transgenes to mitigate abiotic stress in soybean, provided the transgene under control of these promoters does not invoke agronomic penalties with leaky expression when no abiotic stress is imposed.

Original languageEnglish (US)
Pages (from-to)55-64
Number of pages10
JournalPlanta
Volume237
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Dehydration
Soybeans
Arabidopsis
water stress
promoter regions
soybeans
Glucuronidase
Plant Leaves
Droughts
Transgenes
abiotic stress
transgenes
Water Deprivation
drought
Plant Roots
leaves
water deprivation
plant response
Glycine max
Arabidopsis thaliana

Keywords

  • Agrobacterium tumefaciens
  • Drought
  • Gas exchange
  • Glycine max
  • Promoter activity

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

Activity of the Arabidopsis RD29A and RD29B promoter elements in soybean under water stress. / Bihmidine, Saadia; Lin, Jiusheng; Stone, Julie M.; Awada, Tala; Specht, James E.; Clemente, Tom E.

In: Planta, Vol. 237, No. 1, 01.01.2013, p. 55-64.

Research output: Contribution to journalArticle

Bihmidine, Saadia ; Lin, Jiusheng ; Stone, Julie M. ; Awada, Tala ; Specht, James E. ; Clemente, Tom E. / Activity of the Arabidopsis RD29A and RD29B promoter elements in soybean under water stress. In: Planta. 2013 ; Vol. 237, No. 1. pp. 55-64.
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