Activation of the NaCl- and drought-induced RD29A and RD29B promoters by constitutively active Arabidopsis MAPKK or MAPK proteins

Zhi Ming Hua, Xianci Yang, Michael E Fromm

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Mitogen-activated protein (MAP) kinases mediate cellular responses to a wide variety of stimuli. Activation of a MAP kinase (MAPK) occurs after phosphorylation by an upstream MAP kinase kinase (MAPKK). The Arabidopsis thaliana genome encodes 10 MKKs, but few of these have been shown directly to activate any of the 20 Arabidopsis MAPKs (AtMPKs) and NaCl-, drought- or abscisic acid (ABA)-induced genes RD29A or RD29B. We have constructed the coustitutively activated form for nine of the 10 AtMKK proteins, and tested their ability to activate the RD29A and RD29B promoters and also checked the ability of the nine activated AtMKK proteins to phosphorylate 11 of the AtMPK proteins in transient assays. The results show that three proteins, AtMKK1, AtMKK2 and AtMKK3, could activate the RD29A promoter, while these three and two additional AtMKK6/8 proteins could activate the RD29B promoter. Four other proteins, AtMKK7/ AtMKK9 and AtMKK4/AtMKK5, can cause hypersensitive response (HR) in tobacco leaves using transient analysis. The activation of the RD29A promoter correlated with four uniquely activated AtMPK proteins. A novel method of activating AtMPK proteins by fusion to a cis-acting mutant of a human MAPK kinase MEK1 was used to confirm that specific members of the AtMPK gene family can activate the RD29A stress pathway.

Original languageEnglish (US)
Pages (from-to)1761-1770
Number of pages10
JournalPlant, Cell and Environment
Volume29
Issue number9
DOIs
StatePublished - Sep 1 2006

Fingerprint

mitogen-activated protein kinase kinase kinase
MAP Kinase Kinase Kinases
mitogen-activated protein kinase kinase
Droughts
Mitogen-Activated Protein Kinase Kinases
Arabidopsis
Arabidopsis Proteins
promoter regions
drought
Mitogen-Activated Protein Kinases
Proteins
proteins
Abscisic Acid
mitogen-activated protein kinase
Genes
Tobacco
Phosphorylation
Genome
hypersensitive response
abscisic acid

Keywords

  • Arabidopsis thaliana
  • Hypersensitive response

ASJC Scopus subject areas

  • Plant Science

Cite this

Activation of the NaCl- and drought-induced RD29A and RD29B promoters by constitutively active Arabidopsis MAPKK or MAPK proteins. / Hua, Zhi Ming; Yang, Xianci; Fromm, Michael E.

In: Plant, Cell and Environment, Vol. 29, No. 9, 01.09.2006, p. 1761-1770.

Research output: Contribution to journalArticle

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