Activation of the human immunodeficiency virus by herpes simplex virus type 1

J. M. Ostrove, J. Leonard, K. E. Weck, A. B. Rabson, Howard Eliot Gendelman

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Abstract

Herpes simplex virus type 1 (HSV-1) and some of its immediate-early genes stimulate expression of the human immunodeficiency virus (HIV) long terminal repeat (LTR) sequences and the replication of HIV itself. To demonstrate this, the HIV LTR was linked to the indicator gene chloramphenicol acetyltransferase (CAT) and transfected into Vero cells with or without the trans-activating gene (tat) of HIV. Infection of these cells with HSV-1 strain KOS or temperature-sensitive mutant tsB21 or tsE6 resulted in a large increase in CAT activity in the absence of tat and further augmentation in the presence of tat. This stimulation was seen at both their permissive (34°C) and nonpermissive (39°C) temperature, implying either that HSV-1 infection or immediate early gene expression is all that is required. In cotransfection assays in Vero cells, cloned HSV-1 immediate early genes ICP0 and ICP4 stimulated CAT activity in the presence of tat, while ICP27 had no effect. On the other hand, in SW480 cells, ICP4 and, to a lesser extent, ICP0 genes caused stimulation of CAT activity in the absence of tat. Deletion mutants within the HIV LTR showed that the target for HSv stimulation is distinct from the tat-responsive area and maps near the SP1 binding sites. In HeLa cells, ICP0 or ICP4 stimulated the replication of a cotransfected clone of HIV, as shown by an increase in reverse transcriptase activity in the culture supernatant.

Original languageEnglish (US)
Pages (from-to)3726-3732
Number of pages7
JournalJournal of virology
Volume61
Issue number12
StatePublished - Dec 1 1987

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Human herpesvirus 1
Human immunodeficiency virus
Human Herpesvirus 1
HIV
chloramphenicol acetyltransferase
Chloramphenicol O-Acetyltransferase
HIV Long Terminal Repeat
terminal repeat sequences
Genes
Immediate-Early Genes
genes
Vero Cells
cells
Gene Expression
Temperature
Terminal Repeat Sequences
RNA-Directed DNA Polymerase
gene expression
mutants
Virus Diseases

ASJC Scopus subject areas

  • Immunology

Cite this

Ostrove, J. M., Leonard, J., Weck, K. E., Rabson, A. B., & Gendelman, H. E. (1987). Activation of the human immunodeficiency virus by herpes simplex virus type 1. Journal of virology, 61(12), 3726-3732.

Activation of the human immunodeficiency virus by herpes simplex virus type 1. / Ostrove, J. M.; Leonard, J.; Weck, K. E.; Rabson, A. B.; Gendelman, Howard Eliot.

In: Journal of virology, Vol. 61, No. 12, 01.12.1987, p. 3726-3732.

Research output: Contribution to journalArticle

Ostrove, JM, Leonard, J, Weck, KE, Rabson, AB & Gendelman, HE 1987, 'Activation of the human immunodeficiency virus by herpes simplex virus type 1', Journal of virology, vol. 61, no. 12, pp. 3726-3732.
Ostrove JM, Leonard J, Weck KE, Rabson AB, Gendelman HE. Activation of the human immunodeficiency virus by herpes simplex virus type 1. Journal of virology. 1987 Dec 1;61(12):3726-3732.
Ostrove, J. M. ; Leonard, J. ; Weck, K. E. ; Rabson, A. B. ; Gendelman, Howard Eliot. / Activation of the human immunodeficiency virus by herpes simplex virus type 1. In: Journal of virology. 1987 ; Vol. 61, No. 12. pp. 3726-3732.
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