Activation of a cryptic gene cluster in lysobacter enzymogenes reveals a module/domain portable mechanism of nonribosomal peptide synthetases in the biosynthesis of pyrrolopyrazines

Shanren Li, Xiuli Wu, Limei Zhang, Yuemao Shen, Liangcheng Du

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lysobacter are considered "peptide specialists". However, many of the nonribosomal peptide synthetase genes are silent. Three new compounds were identified from L. enzymogenes upon activating the six-module-containing led cluster by the strong promoter PHSAF. Although ledD was the first gene under PHSAF control, the second gene ledE was expressed the highest. Targeted gene inactivation showed that the two-module LedE and the one-module LedF were selectively used in pyrrolopyrazine biosynthesis, revealing a module/domain portable mechanism.

Original languageEnglish (US)
Pages (from-to)5010-5013
Number of pages4
JournalOrganic Letters
Volume19
Issue number19
DOIs
StatePublished - Jan 1 2017

Fingerprint

Lysobacter
Peptide Synthases
biosynthesis
Biosynthesis
Multigene Family
genes
peptides
Genes
modules
Chemical activation
activation
Gene Silencing
deactivation
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

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abstract = "Lysobacter are considered {"}peptide specialists{"}. However, many of the nonribosomal peptide synthetase genes are silent. Three new compounds were identified from L. enzymogenes upon activating the six-module-containing led cluster by the strong promoter PHSAF. Although ledD was the first gene under PHSAF control, the second gene ledE was expressed the highest. Targeted gene inactivation showed that the two-module LedE and the one-module LedF were selectively used in pyrrolopyrazine biosynthesis, revealing a module/domain portable mechanism.",
author = "Shanren Li and Xiuli Wu and Limei Zhang and Yuemao Shen and Liangcheng Du",
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T1 - Activation of a cryptic gene cluster in lysobacter enzymogenes reveals a module/domain portable mechanism of nonribosomal peptide synthetases in the biosynthesis of pyrrolopyrazines

AU - Li, Shanren

AU - Wu, Xiuli

AU - Zhang, Limei

AU - Shen, Yuemao

AU - Du, Liangcheng

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Lysobacter are considered "peptide specialists". However, many of the nonribosomal peptide synthetase genes are silent. Three new compounds were identified from L. enzymogenes upon activating the six-module-containing led cluster by the strong promoter PHSAF. Although ledD was the first gene under PHSAF control, the second gene ledE was expressed the highest. Targeted gene inactivation showed that the two-module LedE and the one-module LedF were selectively used in pyrrolopyrazine biosynthesis, revealing a module/domain portable mechanism.

AB - Lysobacter are considered "peptide specialists". However, many of the nonribosomal peptide synthetase genes are silent. Three new compounds were identified from L. enzymogenes upon activating the six-module-containing led cluster by the strong promoter PHSAF. Although ledD was the first gene under PHSAF control, the second gene ledE was expressed the highest. Targeted gene inactivation showed that the two-module LedE and the one-module LedF were selectively used in pyrrolopyrazine biosynthesis, revealing a module/domain portable mechanism.

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U2 - 10.1021/acs.orglett.7b01611

DO - 10.1021/acs.orglett.7b01611

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