Activation induces a rapid reorganization of spectrin in lymphocytes

John K. Lee, Jennifer D Black, Elizabeth A. Repasky, Ralph T. Kubo, Richard B. Bankert

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Lymphocyte activation results in a rapid reorganization of the cytoskeletal protein spectrin. Immediately following an activation signal, there is fragmentation of a spectrin-rich cytoplasmic structure and subsequent translocation of the fragments to defined areas of the plasma membrane in both antigen-specific T cell hybridomas and lymph node T cells. These dramatic changes have been documented by light and electron microscopic immunolocalization and by immunoblot analysis of plasma membrane-enriched preparations. A T cell hybridoma variant lacking the spectrin-rich cytoplasmic structure of the parental line does not redistribute spectrin and produces little or no IL-2 in response to antigen-dependent activation. This suggests a functional link between spectrin distribution and activation potential. We propose that the cytoplasmic structure functions as an organizing center or reservoir for spectrin that is sensitive to signaling at the cell surface.

Original languageEnglish (US)
Pages (from-to)807-816
Number of pages10
JournalCell
Volume55
Issue number5
DOIs
StatePublished - Dec 2 1988

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Spectrin
Lymphocytes
Chemical activation
Cytoplasmic Structures
T-cells
Hybridomas
Cell membranes
T-Lymphocytes
Cell Membrane
Antigens
Cytoskeletal Proteins
Lymphocyte Activation
Interleukin-2
Lymph Nodes
Electrons
Light

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Activation induces a rapid reorganization of spectrin in lymphocytes. / Lee, John K.; Black, Jennifer D; Repasky, Elizabeth A.; Kubo, Ralph T.; Bankert, Richard B.

In: Cell, Vol. 55, No. 5, 02.12.1988, p. 807-816.

Research output: Contribution to journalArticle

Lee, JK, Black, JD, Repasky, EA, Kubo, RT & Bankert, RB 1988, 'Activation induces a rapid reorganization of spectrin in lymphocytes', Cell, vol. 55, no. 5, pp. 807-816. https://doi.org/10.1016/0092-8674(88)90136-5
Lee, John K. ; Black, Jennifer D ; Repasky, Elizabeth A. ; Kubo, Ralph T. ; Bankert, Richard B. / Activation induces a rapid reorganization of spectrin in lymphocytes. In: Cell. 1988 ; Vol. 55, No. 5. pp. 807-816.
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